转基因鱼及其在基础研究和应用研究中的应用。
Transgenic fish and its application in basic and applied research.
作者信息
Chen T T, Vrolijk N H, Lu J K, Lin C M, Reimschuessel R, Dunham R A
机构信息
Biotechnology Center, University of Connecticut, Storrs, USA.
出版信息
Biotechnol Annu Rev. 1996;2:205-36. doi: 10.1016/s1387-2656(08)70011-5.
Since 1985, transgenic fish have been successfully produced by microinjecting or electroporating desired foreign DNA into unfertilized or newly fertilized eggs using many different fish species. More recently, transgenic fish have also been produced by infecting newly fertilized eggs with pantropic, defective retroviral vectors carrying desired foreign DNA. These transgenic fish can serve as excellent experimental models for basic scientific investigations as well as in biotechnological applications. In this paper, we will review the current status of the transgenic fish research and its potential application in basic and applied research.
自1985年以来，通过将所需的外源DNA显微注射或电穿孔到未受精或新受精的鱼卵中，利用许多不同的鱼类成功培育出了转基因鱼。最近，通过用携带所需外源DNA的泛嗜性、缺陷型逆转录病毒载体感染新受精的鱼卵，也培育出了转基因鱼。这些转基因鱼可作为基础科学研究以及生物技术应用的优秀实验模型。在本文中，我们将综述转基因鱼研究的现状及其在基础研究和应用研究中的潜在应用。
Zotero 插件