Ho W K, Meng Z Q, Lin H R, Poon C T, Leung Y K, Yan K T, Dias N, Che A P, Liu J, Zheng W M, Sun Y, Wong A O
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Biochim Biophys Acta. 1998 Aug 24;1381(3):331-9. doi: 10.1016/s0304-4165(98)00044-0.
A total of five recombinant Bombyx mori nuclear polyhedrosis viruses (BMNPV) carrying the grass carp (Ctenopharyngodon idellus) growth hormone (GH) cDNA were constructed in this study. Two of them were able to express the hormone up to a level of 12 microgram/ml medium when cultured B. mori cells were infected for 4 days. Inoculation of the viruses into silkworm (B. mori) host significantly increased the level of GH achievable. The amount of hormone produced per larva was estimated to be around 1 mg. The recombinant grass carp GH had immunological and biological activities similar to the native hormone. The N-terminal sequence of the recombinant hormone was the same as the native one, indicating that the fish signal peptide was correctly processed by the insect cells. Silkworm powder prepared from larvae infected with the recombinant virus was used as food supplement for fish. Compared with the control, this dietary supplement was effective in increasing the growth rate of juvenile carp.
本研究构建了总共五种携带草鱼（Ctenopharyngodon idellus）生长激素（GH）cDNA的重组家蚕核型多角体病毒（BMNPV）。其中两种病毒在感染培养的家蚕细胞4天后，能够将该激素表达至12微克/毫升培养基的水平。将这些病毒接种到家蚕（B. mori）宿主体内可显著提高可达到的GH水平。估计每条幼虫产生的激素量约为1毫克。重组草鱼GH具有与天然激素相似的免疫和生物学活性。重组激素的N端序列与天然序列相同，表明昆虫细胞正确加工了鱼类信号肽。用感染重组病毒的幼虫制备的蚕粉用作鱼类的食物补充剂。与对照组相比，这种膳食补充剂可有效提高幼鲤的生长速率。
