水杨酸诱导大鼠肝微粒体脂质过氧化。

Salicylic acid-induced lipid peroxidation in rat liver microsomes.

作者信息

Doi H, Masubuchi Y, Narimatsu S, Nishigaki R, Horie T

机构信息

Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Chiba University, Japan.

出版信息

Res Commun Mol Pathol Pharmacol. 1998 Jun;100(3):265-71.

PMID:9730006

Abstract

Rat liver microsomes (1 mg protein/ml) were incubated at 37 degrees C with non-steroidal anti-inflammatory drug, salicylic acid (5 mM) in the presence of 0.2 mM NADPH. The amounts of thiobarbituric acid reactive-substances (TBARS) were increased remarkably by the incubation for 30 min. TBARS formation was dependent on salicylic acid concentration. Salicylic acid-induced lipid peroxidation was not observed in the absence of NADPH. It was also inhibited by the presence of cytochrome P450 inhibitor, SKF-525A. The singlet oxygen's scavengers such as histidine suppressed salicylic acid-induced lipid peroxidation. These results suggested that salicylic acid-induced lipid peroxidation was coupled with the metabolism through cytochrome P450 in rat liver microsomes. The singlet oxygen may be involved in the reaction.

摘要

将大鼠肝微粒体（1毫克蛋白质/毫升）在37摄氏度下与非甾体抗炎药水杨酸（5毫摩尔）一起，在0.2毫摩尔烟酰胺腺嘌呤二核苷酸磷酸（NADPH）存在的情况下孵育。孵育30分钟后，硫代巴比妥酸反应性物质（TBARS）的量显著增加。TBARS的形成取决于水杨酸的浓度。在没有NADPH的情况下未观察到水杨酸诱导的脂质过氧化。细胞色素P450抑制剂SKF-525A的存在也会抑制这种现象。单线态氧的清除剂如组氨酸可抑制水杨酸诱导的脂质过氧化。这些结果表明，水杨酸诱导的脂质过氧化与大鼠肝微粒体中通过细胞色素P450的代谢相关联。单线态氧可能参与了该反应。