Crosbie O M, Norris S, Hegarty J E, O'Farrelly C
The Liver Unit and Education and Research Centre, St. Vincent's Hospital, Dublin, Ireland.
Immunol Invest. 1998 Jul-Sep;27(4-5):237-41. doi: 10.3109/08820139809070897.
Changes in T-lymphocyte subsets have previously been shown to relate to clinical events following liver transplantation and be of prognostic significance following renal transplantation. The aim of this study was to examine T lymphocyte subsets, their activation status and the mean fluorescence intensity of cell surface markers by flow cytometric analysis, in peripheral blood of patients following liver transplantation. Stable transplant patients (n=11) had a significantly higher level of activation (HLA-DR expression ) of all T cell subsets: CD3, CD4 and CD8 compared to healthy controls: 17.5% +/- 14.0 (mean +/- SD) vs 4.7 +/- 1.8 (p=0.04), 13.7% +/- 10.3 vs 4.3 +/- 1.7 (p=0.03) and 23.8% +/- 19.9 vs 3.6 +/- 2.4 (p=0.02) respectively. A further increase in activation status occurred in all T cell subsets in association with acute cellular rejection, reaching significance for the CD4+ population: 13.7% +/- 10.2 vs 23.3% +/- 20.6 (p=0.04). The mean fluorescence intensity of the CD3+DR- and CD3+ DR+ populations were increased to 1397 +/- 869 and 1282 +/- 810 following liver transplantation compared to values of 425 +/- 204 and 376 +/- 166 respectively for controls (p<0.05). T-lymphocytes maintain a high level of activation following liver transplantation and continue to express high levels of the surface marker CD3, which may account for the occurrence of acute cellular rejection despite immunosuppression in these patients.
此前已有研究表明，T淋巴细胞亚群的变化与肝移植后的临床事件相关，且对肾移植后的预后具有重要意义。本研究旨在通过流式细胞术分析肝移植患者外周血中的T淋巴细胞亚群、其激活状态以及细胞表面标志物的平均荧光强度。与健康对照组相比，稳定的移植患者（n = 11）所有T细胞亚群（CD3、CD4和CD8）的激活水平（HLA-DR表达）显著更高：分别为17.5%±14.0（平均值±标准差）对4.7±1.8（p = 0.04）、13.7%±10.3对4.3±1.7（p = 0.03）和23.8%±19.9对3.6±2.4（p = 0.02）。在急性细胞排斥反应时，所有T细胞亚群的激活状态进一步升高，CD4+群体达到显著水平：13.7%±10.2对23.3%±20.6（p = 0.04）。肝移植后，CD3+DR-和CD3+DR+群体的平均荧光强度分别增加到1397±869和1282±810，而对照组的值分别为425±204和376±166（p<0.05）。肝移植后T淋巴细胞维持高水平的激活，并持续高表达表面标志物CD3，这可能是这些患者尽管接受了免疫抑制仍发生急性细胞排斥反应的原因。
