Crosbie O M, Costello P J, O'Farrelly C, Hegarty J E
Liver Unit and Education and Research Centre, St. Vincent's Hospital, Elm Park, Dublin, Ireland.
Liver Transpl Surg. 1998 Mar;4(2):141-5. doi: 10.1002/lt.500040207.
Circulating CD3+ T lymphocytes that express neither the CD4 nor CD8 surface molecules (double-negative T lymphocytes) are phenotypically and functionally distinct from single-positive CD3+CD4+ and CD3+CD8+ lymphocytes and are thought to represent a distinct T-cell lineage. The presence of low numbers of double-negative T cells in healthy individuals and the increase observed in association with lymphoproliferative disorders, graft-versus-host disease, and autoimmune diseases suggest a pathogenic or immunoregulatory role for this population of T lymphocytes. In this study, peripheral blood double-negative T cells were assessed quantitatively using three-color flow cytometry in 10 patients after liver transplantation during a 6-week period. During this time, 12 episodes of histologically proven acute cellular rejection occurred in 8 patients. The median postoperative baseline double-negative T-cell count expressed as a proportion of the CD3+ T cells was 2.4 +/- 1.2 (median +/- SD; n = 10), which was identical to a control group of healthy adults (2.5 +/- 2.4; n = 9). Circulating numbers of double-negative T cells were increased significantly during acute cellular rejection (6.8 +/- 6.7; P < .001; n = 12). After pulse corticosteroid therapy for rejection, there was a significant decrease in the double-negative T-cell population (3.5 +/- 5.0 v 6.8 +/- 6.7; P = .01). No significant changes occurred in the double-negative T-cell count in the absence of clinical events (2.4 +/- 3.5; n = 73). These findings are consistent with a role for double-negative T cells in the initiation of acute cellular rejection or a possible regulatory role in the immunologic changes associated with rejection.
循环中的CD3⁺ T淋巴细胞既不表达CD4也不表达CD8表面分子（双阴性T淋巴细胞），在表型和功能上与单阳性CD3⁺CD4⁺和CD3⁺CD8⁺淋巴细胞不同，被认为代表一种独特的T细胞谱系。健康个体中存在少量双阴性T细胞，并且在淋巴增殖性疾病、移植物抗宿主病和自身免疫性疾病中观察到其数量增加，这表明这群T淋巴细胞具有致病或免疫调节作用。在本研究中，使用三色流式细胞术对10例肝移植患者在6周期间的外周血双阴性T细胞进行了定量评估。在此期间，8例患者发生了12次经组织学证实的急性细胞排斥反应。术后双阴性T细胞计数的中位数以占CD3⁺ T细胞的比例表示为2.4±1.2（中位数±标准差；n = 10），这与健康成年人对照组（2.5±2.4；n = 9）相同。在急性细胞排斥反应期间，双阴性T细胞的循环数量显著增加（6.8±6.7；P <.001；n = 12）。在针对排斥反应进行脉冲皮质类固醇治疗后，双阴性T细胞群体显著减少（3.5±5.0对6.8±6.7；P =.01）。在没有临床事件的情况下，双阴性T细胞计数没有显著变化（2.4±3.5；n = 73）。这些发现与双阴性T细胞在急性细胞排斥反应起始中的作用或在与排斥反应相关的免疫变化中的可能调节作用一致。
