Varnado M, Tucci M, Cason Z, Mohamed A, Benghuzzi H
University of Mississippi Medical Center, Jackson 39216, USA.
Biomed Sci Instrum. 1997;33:161-5.
It is well documented that testosterone stimulates protein anabolism, muscular development, bone growth and increased metabolic rate. In addition, exogenous low levels of testosterone can inhibit the secretion of gonadotropins by the anterior pituitary. The objective of this study was to investigate the effect of sustained delivery of testosterone enanthate (TE) on the testicular architecture by means of tricalcium phosphate-lysine(TCPL). In this experiment adult male Sprague Dawley rats (250-300 g BW) were randomly divided into three equal groups (n = 16). Rats in group I were implanted subcutaneously with TE loaded TCPL implants (4-6 ng/ml). Rats in group II were implanted with sham TCPL capsules, and rats in group III served as intact unimplanted controls. Surgical aseptic techniques were performed according to standard laboratory procedures. At the end of 2 and 4 weeks post implantation, eight animals from each group were sacrificed and the testes were collected, weighted, and embedded for histopathological evaluations. The results of this study revealed the following: (i) Sustained delivery of TE reduced spermatogonia numbers in animals after 2 and 4 weeks by approximately 10% in comparison to control animals. (ii) The lumen of the tubules showed slight regression after 4 weeks of TE treatment. (iii) Overall decrease of the vasculature of the experimental animals was seen after 2 and continued through 4 weeks with an occasional increase of microvasculature seen in animals treated for 4 weeks with TE. (iv) Finally, the germinal epithelium showed significant reduction in secondary spermatocytes and conversion to mature sperm.
有充分的文献记载，睾酮能刺激蛋白质合成代谢、肌肉发育、骨骼生长并提高代谢率。此外，外源性低水平的睾酮可抑制垂体前叶促性腺激素的分泌。本研究的目的是通过磷酸三钙 - 赖氨酸（TCPL）研究庚酸睾酮（TE）持续给药对睾丸结构的影响。在本实验中，成年雄性Sprague Dawley大鼠（体重250 - 300克）被随机分为三组，每组16只。第一组大鼠皮下植入负载TE的TCPL植入物（4 - 6纳克/毫升）。第二组大鼠植入假TCPL胶囊，第三组大鼠作为完整未植入的对照。手术无菌技术按照标准实验室程序进行。植入后2周和4周结束时，每组处死8只动物，收集睾丸，称重，并进行组织病理学评估。本研究结果如下：（i）与对照动物相比，TE持续给药2周和4周后，动物精原细胞数量减少约10%。（ii）TE治疗4周后，小管腔出现轻微萎缩。（iii）实验动物的血管在2周后总体减少，并持续到4周，在接受TE治疗4周的动物中偶尔可见微血管增加。（iv）最后，生精上皮的次级精母细胞显著减少并转化为成熟精子。
