Coleman A, Tucci M, Cason Z, Benghuzzi H
University of Mississippi Medical Center, Jackson 39216, USA.
Biomed Sci Instrum. 1997;34:36-40.
Recent studies reported from our laboratory have established that the sustained delivery of danazol in combination with androgens resulted in the remarkable reduction of epididymal mass. In addition, previous studies have recommended that ultrastructural of epididymal tubules have to be elucidated. The specific objective of this investigation was to evaluate the cytological characteristics of epididymal tissues exposed to sustained delivery of dihydrotestosterone (DHT), dehydroepiandrsterone (DHEA) and a combination of Estrogen (E), DHEA plus DHT by means of tricalcium phosphate lysine (TCPL) delivery system. Adult male rats (BW 300-350 gm) were randomly divided into four equal groups: Group I animals were implanted i.p. with TCPL loaded with DHEA (100 mg). Animals in group II were implanted with TCPL capsules loaded with DHEA (100 mg) + DHT (500 mg). Group III animals were implanted with TCPL capsules loaded with E (200 mg) + DHEA (100 mg) + DHT (500 mg). Group IV animals served as the intact unimplanted controls. Surgical aseptic techniques were performed according to standard laboratory procedures. The animals were maintained at the University of Mississippi Medical Center Animal Facilities following the rules and regulations established by NIH on the Care and Use of Laboratory animals. At the end of 6 weeks post implantation, all animals were sacrificed and the epididymal tissues were collected, weighed, and embedded for histopathological evaluations. Statistical analysis was conducted by using standard computer programs (STATVIEW, ANOVA at 95% CI). The data obtained in this investigation demonstrated the following: (1) remarkable reduction in sperm counts and motility obtained from epididymal tubules in all experimental (hormonally treated) groups, (2) the lumen of the epididymal tubules were devoid of sperm in animals treated with DHT in comparison to the control, (3) a decrease in the diameter of tubules with occasional hypertrophic epithelium in all experimental animals, (4) disorganization of nuclear material was observed in animals treated with DHEA and DHEA + E + DHT in comparison to the control group. The overall observation of this study suggests that sustained delivery of DHEA, DHEA + DHT, and DHEA + DHT + E can be used to regulate the structural and functional architecture of the site of extramaturation of spermatozoa.
我们实验室近期发表的研究表明，达那唑与雄激素联合持续给药可显著减轻附睾重量。此外，以往研究建议对附睾管的超微结构进行阐释。本研究的具体目的是通过磷酸三钙赖氨酸（TCPL）给药系统，评估暴露于双氢睾酮（DHT）、脱氢表雄酮（DHEA）以及雌激素（E）、DHEA加DHT联合持续给药后的附睾组织的细胞学特征。成年雄性大鼠（体重300 - 350克）被随机分为四组，每组数量相等：第一组动物经腹腔注射植入载有DHEA（100毫克）的TCPL。第二组动物植入载有DHEA（100毫克）+ DHT（500毫克）的TCPL胶囊。第三组动物植入载有E（200毫克）+ DHEA（100毫克）+ DHT（500毫克）的TCPL胶囊。第四组动物作为完整未植入的对照组。按照标准实验室程序实施手术无菌技术。这些动物在密西西比大学医学中心动物设施中饲养，遵循美国国立卫生研究院制定的关于实验动物饲养和使用的规则与条例。植入后6周结束时，处死所有动物，收集附睾组织，称重，并进行包埋以进行组织病理学评估。使用标准计算机程序（STATVIEW，95%置信区间的方差分析）进行统计分析。本研究获得的数据表明：（1）所有实验（激素处理）组的附睾管精子数量和活力显著降低；（2）与对照组相比，用DHT处理的动物附睾管腔中没有精子；（3）所有实验动物的小管直径减小，偶尔出现上皮肥大；（4）与对照组相比，用DHEA以及DHEA + E + DHT处理的动物观察到核物质紊乱。本研究的总体观察结果表明，DHEA、DHEA + DHT以及DHEA + DHT + E的持续给药可用于调节精子体外成熟部位的结构和功能结构。
