Raleigh J A, Calkins-Adams D P, Rinker L H, Ballenger C A, Weissler M C, Fowler W C, Novotny D B, Varia M A
Department of Radiation Oncology, University of North Carolina School of Medicine, Chapel Hill 27599, USA.
Cancer Res. 1998 Sep 1;58(17):3765-8.
Hypoxia in human tumors is associated with poor prognosis, but the molecular mechanisms underlying this association are poorly understood. One possibility is that hypoxia is linked to malignant progression through vascular endothelial growth factor (VEGF) induction and the associated angiogenesis and metastasis. The present clinical study measures hypoxia and VEGF expression on a cell-by-cell basis in human squamous cell carcinomas to test the hypothesis that hypoxia and VEGF protein expression are coupled in human tumors. Eighteen patients with invasive squamous cell carcinoma of the uterine cervix and head and neck have been investigated by a quantitative image analysis of immunostained sections from their tumors. The hypoxia marker pimonidazole was used to measure tumor hypoxia, and a commercially available antibody was used to measure VEGF protein expression. A quantitative immunohistochemical comparison of hypoxia and VEGF protein expression revealed no correlation between the two factors.
人类肿瘤中的缺氧与预后不良相关，但这种关联背后的分子机制却知之甚少。一种可能性是，缺氧通过诱导血管内皮生长因子（VEGF）以及相关的血管生成和转移与恶性进展相关联。本临床研究在人鳞状细胞癌中逐细胞测量缺氧和VEGF表达，以检验缺氧与VEGF蛋白表达在人类肿瘤中相互关联的假设。通过对18例子宫颈、头颈部浸润性鳞状细胞癌患者肿瘤免疫染色切片进行定量图像分析，对其进行了研究。缺氧标志物匹莫硝唑用于测量肿瘤缺氧情况，一种市售抗体用于测量VEGF蛋白表达。对缺氧和VEGF蛋白表达进行的定量免疫组化比较显示，这两个因素之间没有相关性。
