细胞色素c中展开和重新折叠途径的证据。
Evidence for an unfolding and refolding pathway in cytochrome c.
作者信息
Xu Y, Mayne L, Englander S W
机构信息
Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104, USA.
出版信息
Nat Struct Biol. 1998 Sep;5(9):774-8. doi: 10.1038/1810.
It has been suggested that three partially unfolded forms detected in a native state hydrogen exchange study of oxidized cytochrome c may represent sequential intermediates in an unfolding-refolding pathway. To better define the structure of each intermediate a 'stability labeling' method was used in which the stability of a given segment against unfolding was changed. The condition--folded or unfolded--of the stability-labeled segment in each intermediate could then be determined. The structures found are discrete and native-like and are just those necessary for a sequential pathway.
有人提出，在氧化型细胞色素c的天然态氢交换研究中检测到的三种部分展开形式可能代表了一条去折叠-再折叠途径中的连续中间体。为了更好地定义每个中间体的结构，使用了一种“稳定性标记”方法，其中改变给定片段抵抗去折叠的稳定性。然后可以确定每个中间体中稳定性标记片段的状态——折叠或未折叠。所发现的结构是离散的且类似天然结构，并且正是连续途径所必需的那些结构。
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