Aceituno E, Turrión F, San Román J M, Sarasa J L, Ortiz F, García R
Departamento de Inmunología, Fundación Jiménez Díaz, Madrid.
Med Clin (Barc). 1998 Jul 11;111(5):168-71.
The variant v6 of CD44 has been associated with metastatic behaviour in neoplasms such as lymphoma, colon carcinoma and breast carcinoma. The expression of CD44v6 in breast carcinoma by flow cytometry and its relationship with other tumor markers is studied in this paper.
The expression of CD44v6 was studied in 46 fresh tissue specimens by flow cytometry using a monoclonal antibody which recognizes the variant v6. Ploidy and cell cycle were also studied by flow-cytometry using propidium iodide labelling. p 53, c-erbB-2 and estrogen receptor expression as well as cell proliferation by Ki-67 staining were performed by immunohistochemistry.
Nineteen out of 46 tumors were CD44v6 positive, without correlation with other tumor markers. Levels of CD44v6 in 19 positive samples sligtly correlates with S-phase and hystological grade.
In the present study there was not a correlation among the presence of the variant v6 of CD44 on tumor cells from breast carcinoma and the aggressivity of the tumor. The expression of CD44v6 by flow cytometry does not seem to be a good prognostic marker.
CD44的v6变体已被证实与淋巴瘤、结肠癌和乳腺癌等肿瘤的转移行为有关。本文通过流式细胞术研究了CD44v6在乳腺癌中的表达及其与其他肿瘤标志物的关系。
使用识别v6变体的单克隆抗体，通过流式细胞术研究46份新鲜组织标本中CD44v6的表达。还使用碘化丙啶标记，通过流式细胞术研究倍性和细胞周期。通过免疫组织化学检测p53、c-erbB-2和雌激素受体表达以及Ki-67染色检测细胞增殖情况。
46例肿瘤中有19例CD44v6呈阳性，与其他肿瘤标志物无相关性。19份阳性样本中CD44v6水平与S期和组织学分级略有相关。
在本研究中，乳腺癌肿瘤细胞中CD44的v6变体的存在与肿瘤的侵袭性之间没有相关性。通过流式细胞术检测CD44v6的表达似乎不是一个良好的预后标志物。
