Benoist J F, Orbach D, Biou D
Service de Biochimie-Hormonologie, Centre Hospitalier Robert Debre, Paris, France.
Clin Chem. 1998 Sep;44(9):1980-5.
Increased serum C-reactive protein (sCRP) is a sensitive marker of renal graft rejection. We describe the cases of two children with uncomplicated renal transplantation who had false-positive sCRP values on analyzers using rabbit anti-CRP but values within the reference range with anti-CRP from other animal species. Cross-reaction with heterophilic antibodies was suggested by clinical and biological signs of serum sickness and daily treatment with rabbit antilymphocyte globulin (ALG). The interference depended on the serum concentration of the cross-reactant and was removed by subtotal IgG adsorption to Protein A or Protein G or by immunoadsorption using rabbit ALG or total IgG in non-immune rabbit serum. Anti-rabbit IgG and IgM antibodies were detected in both patients. These are the first reported cases of cross-reaction with heterophilic antibodies in a turbidimetric CRP assay.
血清C反应蛋白（sCRP）升高是肾移植排斥反应的敏感标志物。我们描述了两名接受单纯肾移植的儿童病例，他们在使用兔抗C反应蛋白的分析仪上sCRP值呈假阳性，但使用其他动物来源的抗C反应蛋白时，其值在参考范围内。血清病的临床和生物学体征以及每日使用兔抗淋巴细胞球蛋白（ALG）治疗提示存在与嗜异性抗体的交叉反应。这种干扰取决于交叉反应物的血清浓度，通过用蛋白A或蛋白G进行部分IgG吸附，或使用兔ALG或非免疫兔血清中的总IgG进行免疫吸附可消除干扰。在两名患者中均检测到抗兔IgG和IgM抗体。这些是首次报道的在比浊法C反应蛋白检测中与嗜异性抗体发生交叉反应的病例。
