用异源DNA对莱姆病螺旋体伯氏疏螺旋体进行转化。
Transformation of the Lyme disease spirochete Borrelia burgdorferi with heterologous DNA.
作者信息
Stevenson B, Bono J L, Elias A, Tilly K, Rosa P
机构信息
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
出版信息
J Bacteriol. 1998 Sep;180(18):4850-5. doi: 10.1128/JB.180.18.4850-4855.1998.
Abstract
Studies of the spirochete Borrelia burgdorferi have been hindered by the scarcity of genetic tools that can be used in these bacteria. For the first time, a method has been developed by which heterologous DNA (DNA without a naturally occurring B. burgdorferi homolog) can be introduced into and persistently maintained by B. burgdorferi. This technique uses integration of circular DNA into the bacterial genome via a single-crossover event. The ability to transform B. burgdorferi with heterologous DNA will now permit a wide range of experiments on the biology of these bacteria and their involvement in the many facets of Lyme disease.
摘要
对伯氏疏螺旋体的研究一直受到可用于这些细菌的遗传工具稀缺的阻碍。首次开发出了一种方法，通过该方法可将异源DNA（没有天然存在的伯氏疏螺旋体同源物的DNA）导入伯氏疏螺旋体并使其持续维持。该技术利用环状DNA通过单交换事件整合到细菌基因组中。用异源DNA转化伯氏疏螺旋体的能力现在将允许对这些细菌的生物学特性及其在莱姆病诸多方面的作用进行广泛的实验。
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本文引用的文献
1
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Infect Immun. 1998 Jun;66(6):2648-54. doi: 10.1128/IAI.66.6.2648-2654.1998.
2
Oligopeptide permease in Borrelia burgdorferi: putative peptide-binding components encoded by both chromosomal and plasmid loci.伯氏疏螺旋体中的寡肽通透酶：由染色体和质粒位点编码的假定肽结合成分。
Microbiology (Reading). 1998 Apr;144 ( Pt 4):1033-1044. doi: 10.1099/00221287-144-4-1033.
3
Analysis of promoters in Borrelia burgdorferi by use of a transiently expressed reporter gene.利用瞬时表达报告基因分析伯氏疏螺旋体中的启动子。
J Bacteriol. 1997 Nov;179(21):6837-42. doi: 10.1128/jb.179.21.6837-6842.1997.
4
The Borrelia burgdorferi circular plasmid cp26: conservation of plasmid structure and targeted inactivation of the ospC gene.伯氏疏螺旋体环状质粒cp26：质粒结构的保守性及ospC基因的靶向失活
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5
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6
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7
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8
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10
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