对钠离子有反应的ntp操纵子对于在有限质子电势下平肠球菌中钾离子和钠离子的稳态不可或缺。
The Na+-responsive ntp operon is indispensable for homeostatis of K+ and Na+ in Enterococcus hirae at limited proton potential.
作者信息
Kawano M, Igarashi K, Kakinuma Y
机构信息
Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan.
出版信息
J Bacteriol. 1998 Sep;180(18):4942-5. doi: 10.1128/JB.180.18.4942-4945.1998.
Abstract
Enterococcus hirae ATCC 9790 grew well in Na+-deficient, low-K+ medium, but growth was inhibited by carbonylcyanide m-chlorophenylhydrazone (CCCP). Growth inhibition and decrease of cellular K+ levels in the presence of CCCP were relieved by the addition of Na+ and a high concentration of K+. In contrast, in the mutant defective in Na+-ATPase or the NtpJ component of the KtrII K+ uptake system, CCCP-induced growth inhibition was rescued by a high concentration of K+ but not of Na+. These transporters are thus indispensable for homeostatis of K+ and Na+ at low proton potential.
摘要
平肠球菌ATCC 9790在缺钠、低钾培养基中生长良好，但生长受到羰基氰化物间氯苯腙（CCCP）的抑制。添加Na⁺和高浓度的K⁺可缓解CCCP存在时的生长抑制和细胞K⁺水平降低。相比之下，在Na⁺-ATP酶或KtrII K⁺摄取系统的NtpJ组分有缺陷的突变体中，高浓度的K⁺而非Na⁺可挽救CCCP诱导的生长抑制。因此，这些转运蛋白对于低质子势下K⁺和Na⁺的稳态是不可或缺的。
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