通过锤头状核酶切割在体内监测逆转录病毒RNA二聚化
Monitoring retroviral RNA dimerization in vivo via hammerhead ribozyme cleavage.
作者信息
Pal B K, Scherer L, Zelby L, Bertrand E, Rossi J J
机构信息
Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA.
出版信息
J Virol. 1998 Oct;72(10):8349-53. doi: 10.1128/JVI.72.10.8349-8353.1998.
Abstract
We have used a strategy for colocalization of Psi (Psi)-tethered ribozymes and targets to demonstrate that Psi sequences are capable of specific interaction in the cytoplasm of both packaging and nonpackaging cells. These results indicate that current in vitro dimerization models may have in vivo counterparts. The methodology used may be applied to further genetic analyses on Psi domain interactions in vivo.
摘要
我们采用了一种将 Psi（Psi）-连接的核酶与靶标共定位的策略，以证明 Psi 序列能够在包装细胞和非包装细胞的细胞质中进行特异性相互作用。这些结果表明，当前的体外二聚化模型可能在体内存在对应物。所使用的方法可应用于进一步对 Psi 结构域在体内的相互作用进行遗传分析。
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