人类巨细胞病毒β2.7转录本的主要开放阅读框：一种受5'区域转录后调控的蛋白质的体外表达
The major open reading frame of the beta2.7 transcript of human cytomegalovirus: in vitro expression of a protein posttranscriptionally regulated by the 5' region.
作者信息
Bergamini G, Reschke M, Battista M C, Boccuni M C, Campanini F, Ripalti A, Landini M P
机构信息
Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, St. Orsola Hospital, Bologna, Italy.
出版信息
J Virol. 1998 Oct;72(10):8425-9. doi: 10.1128/JVI.72.10.8425-8429.1998.
Abstract
beta2.7 is the major early transcript produced during human cytomegalovirus infection. This abundantly expressed RNA is polysome associated, but no protein product has ever been detected. In this study, a stable peptide of 24 kDa was produced in vitro from the major open reading frame (ORF), TRL4. Following transient transfection, the intracellular localization was nucleolar and the expression was posttranscriptionally inhibited by the 5' sequence of the transcript, which harbors two short upstream ORFs.
摘要
β2.7是人类巨细胞病毒感染期间产生的主要早期转录本。这种大量表达的RNA与多核糖体相关，但从未检测到蛋白质产物。在本研究中，从主要开放阅读框（ORF）TRL4体外产生了一种24 kDa的稳定肽。瞬时转染后，细胞内定位在核仁，并且转录本的5'序列对表达具有转录后抑制作用，该序列含有两个短的上游开放阅读框。
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