Huang C, Wang G, Lin H, Li S
Department of Biochemistry and Molecular Genetics, Health Sciences Center, University of Virginia, Box 440, Charlottesville, VA 22908, USA.
Biochim Biophys Acta. 1998 Aug 14;1373(1):282-8. doi: 10.1016/s0005-2736(98)00092-3.
We have determined calorimetrically the phase transition temperature (Tm) values of five sn-1 saturated/sn-2 unsaturated phosphatidylethanolamines (PE) in which the sn-1 acyl chain has 20 carbons and the sn-2 acyl chain has 18 carbons with different number and position of the cis double bond. When these Tm values are combined with the five published Tm values of related unsaturated PE, a unifying Tm diagram is generated for the first time. Moreover, as the molecular mechanics simulated structures of these lipids are taken into consideration, this unifying Tm diagram provides insight into how variations in the number and position of the cis double bond in the lipid's sn-2 acyl chain can influence the phase transition behavior of the lipid bilayer.
我们通过量热法测定了五种sn-1饱和/sn-2不饱和磷脂酰乙醇胺（PE）的相变温度（Tm）值，其中sn-1酰基链含有20个碳原子，sn-2酰基链含有18个碳原子，且顺式双键的数量和位置各不相同。当将这些Tm值与已发表的五种相关不饱和PE的Tm值相结合时，首次生成了一个统一的Tm图。此外，考虑到这些脂质的分子力学模拟结构，这个统一的Tm图有助于深入了解脂质sn-2酰基链中顺式双键的数量和位置变化如何影响脂质双层的相变行为。
