Czerwiecki L
Zakład Analizy Zywności, Instytut Biotechnologii Przemysłu Rolno-Spozywczego, Warszawa.
Rocz Panstw Zakl Hig. 1998;49(1):13-24.
The aim of this study was to perform a optimised method for determination of fumonisins B1 and B2 in corn products. The manner of extraction and clean-up of corn products extracts as well conditions of reaction of fumonisins with OPA was described. The main steps of optimised analytical procedure were: extraction of sample with methanol and water (3 + 1), clean-up of extracts on SAX column, derivatisation with OPA, and determination by means of RP-HPLC. The mobile phase was a mixture of methanol, water and acetic acid (75 + 24 + 1). Fluorometric detection was made at 370/440 nm. The mean recovery of fumonisins dependent on fortification level and product was 64-95%, limit of detection for each of fumonisins was 15 micrograms/kg.
本研究的目的是建立一种优化方法，用于测定玉米制品中的伏马菌素B1和B2。描述了玉米制品提取物的提取和净化方式以及伏马菌素与邻苯二甲醛（OPA）的反应条件。优化分析程序的主要步骤为：用甲醇和水（3+1）提取样品，在强阴离子交换（SAX）柱上净化提取物，用OPA衍生化，并通过反相高效液相色谱（RP-HPLC）进行测定。流动相为甲醇、水和乙酸的混合物（75+24+1）。荧光检测在370/440nm波长下进行。伏马菌素的平均回收率取决于强化水平和产品，为64%-95%，每种伏马菌素的检测限为15微克/千克。
