Slomski R
Ann Med Sect Pol Acad Sci. 1976(3):139-49.
Electrophoretic studies on nuclear RNA extracted from the spleens of immunized mice are reported. RNA was extracted by the phenol-detergent procedure from the purified nuclei of spleen cells at the peak of primary immune response after a single immunization with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis of the ribosomal RNA precursors, 28S, 18S, 5S and 4S fractions of nuclear RNA in the spleens of immunized mice.
本文报道了对从免疫小鼠脾脏中提取的核RNA的电泳研究。在用绵羊红细胞单次免疫后，在初次免疫反应高峰期，通过酚 - 去污剂法从纯化的脾细胞核中提取RNA。用32P - 正磷酸盐进行体内标记的动力学表明，免疫小鼠脾脏中核RNA的核糖体RNA前体28S、18S、5S和4S组分的生物合成加速。
