一种用于测定动物组织中黄曲霉毒素残留量的化学检测方法。
A chemical assay method for the determination of aflatoxin residues in animal tissues.
作者信息
Jemmali M, Murthy T R
出版信息
Z Lebensm Unters Forsch. 1976;161(1):13-7. doi: 10.1007/BF01145414.
A method is presented for the determination of aflatoxins in animal tissues. It consists of extraction of aflatoxins from the sample with methanol, treatment of the residue with a mixture of dimethoxymethane-methanol (4+1) to further precipitate proteins, evaporation of dimethoxymethane followed by a liquid-liquid defatting with hexane and heating of the methanol-water mixture before the transfer of aflatoxins into chloroform. Chloroform extracts are further purified by silica gel--acidic alumina--anhydrous sodium sulfate column chromatography.
介绍了一种测定动物组织中黄曲霉毒素的方法。该方法包括用甲醇从样品中提取黄曲霉毒素，用二甲氧基甲烷 - 甲醇（4 + 1）混合物处理残渣以进一步沉淀蛋白质，蒸去二甲氧基甲烷，然后用己烷进行液 - 液脱脂，并在将黄曲霉毒素转移至氯仿之前加热甲醇 - 水混合物。氯仿提取物通过硅胶 - 酸性氧化铝 - 无水硫酸钠柱色谱法进一步纯化。
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