Miller N, Pretorius H E, Trinder D W
J Assoc Off Anal Chem. 1985 Jan-Feb;68(1):136-7.
A simple method is proposed for determination of aflatoxins in vegetable oils. The method was successfully applied to both crude and degummed oils. The oil sample, dissolved in hexane, was applied to a silica column and washed with ether, toluene, and chloroform; aflatoxins were eluted from the column with chloroform-methanol (97 + 3). As quantitated by thin layer chromatography and liquid chromatography, the oils analyzed contained aflatoxin B1 at levels of 5-200 micrograms/kg. Recoveries of aflatoxin B1 standards added to aflatoxin-free oils were between 89.5 and 93.5%, with coefficients of variation of 6.3-8.0%.
提出了一种测定植物油中黄曲霉毒素的简单方法。该方法已成功应用于毛油和脱胶油。将溶解在己烷中的油样应用于硅胶柱，并用乙醚、甲苯和氯仿洗涤；用氯仿 - 甲醇（97 + 3）从柱上洗脱黄曲霉毒素。通过薄层色谱法和液相色谱法定量，分析的油中黄曲霉毒素B1的含量为5 - 200微克/千克。添加到不含黄曲霉毒素的油中的黄曲霉毒素B1标准品的回收率在89.5%至93.5%之间，变异系数为6.3 - 8.0%。
