Ito K, Tanaka E, Mori H, Nakazawa H, Tanino R
Department of Plastic and Reconstructive Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Plast Reconstr Surg. 1998 Sep;102(4):1128-33. doi: 10.1097/00006534-199809040-00032.
Conventional angiography cannot resolve dermal small vessels with a diameter of 200 microm or less. In vitro microangiography is currently characterized by better spatial resolution than conventional angiography but does not allow visualization of the blood stream in dermal vessels in vivo. In this study, we introduce a novel synchrotron radiation microangiographic system for visualizing the structure of and blood flow in dermal microvessels in vivo repeatedly. We used monochromatic synchrotron radiation with an energy just above the k-edge of iodine (33.3 keV) as an x-ray and a high-definition television camera system with a high-sensitivity image pick-up tube for detection. The 33.3-keV monochromatic synchrotron radiation allows detection of a small amount of iodine, and the high-definition television camera system can resolve small vessels with high-spatial resolution and no loss of sensitivity. We performed synchrotron radiation angiography of superficial inferior epigastric arteries and their branches in nine rats, and of the caudal artery in 14 rats, and compared angiographic images taken by the current system with those taken by a conventional angiographic system in seven rats. With this new microangiographic technique, we could visualize small dermal vessels with a diameter as low as 50 microm. In addition, repeated angiograms at baseline and under increasing body temperature could be obtained. This new microangiographic approach is expected to be very useful for the assessment of dermal circulation in patients.
传统血管造影术无法分辨直径200微米及以下的真皮小血管。目前，体外微血管造影术的空间分辨率优于传统血管造影术，但其无法在体内观察真皮血管内的血流情况。在本研究中，我们引入了一种新型同步辐射微血管造影系统，用于反复观察体内真皮微血管的结构和血流情况。我们使用能量略高于碘的K吸收边（33.3 keV）的单色同步辐射作为X射线，并使用带有高灵敏度摄像管的高清电视摄像系统进行检测。33.3 keV的单色同步辐射能够检测到少量碘，而高清电视摄像系统能够以高空间分辨率分辨小血管，且不失灵敏度。我们对9只大鼠的腹壁下浅动脉及其分支，以及14只大鼠的尾动脉进行了同步辐射血管造影，并将本系统拍摄的血管造影图像与7只大鼠的传统血管造影系统拍摄的图像进行了比较。通过这种新的微血管造影技术，我们能够观察到直径低至50微米的真皮小血管。此外，还能够在基线和体温升高时获取重复的血管造影图像。这种新的微血管造影方法有望在评估患者真皮循环方面非常有用。
