Thomson A M, Roberts N A, Wood W G
MRC Molecular Haematology Unit, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital.
Br J Haematol. 1998 Sep;102(4):976-85. doi: 10.1046/j.1365-2141.1998.00864.x.
We have characterized a murine hybrid cell line, Bb1-3, generated by the fusion of mouse primary erythroblasts with MEL cells. It proliferated in serum-free medium and displayed a low level of spontaneous erythroid and megakaryocyte differentiation. Terminal erythroid differentiation could be induced with HMBA and DMSO and was enhanced by serum. Treatment with phorbol esters resulted in a high proportion of megakaryocytes and the expression of megakaryocytic specific lineage markers. Bb1-3 cells contain a human beta-globin transgene that was expressed at levels of 20-50% of the endogenous mouse globin genes. Initially, expression was largely limited to the beta-globin gene but after adaptation to serum free growth, equal expression of both the human gamma- and human beta-globin genes was observed. This cell line provides further evidence that the differentiation potential of mouse erythroleukaemia cells is not restricted to the erythroid lineage and should be useful to study the mechanisms underlying both developmental globin gene regulation and the terminal differentiation of bipotential erythroid/megakaryocytic progenitor cells.
我们已鉴定出一种小鼠杂交细胞系Bb1-3，它是由小鼠原代成红细胞与MEL细胞融合产生的。该细胞系在无血清培养基中增殖，并表现出低水平的自发红系和巨核系分化。用六亚甲基双乙酰胺（HMBA）和二甲基亚砜（DMSO）可诱导终末红系分化，血清可增强这种分化。佛波酯处理导致高比例的巨核细胞生成及巨核细胞特异性谱系标志物的表达。Bb1-3细胞含有一个人β-珠蛋白转基因，其表达水平为内源性小鼠珠蛋白基因的20%-50%。最初，表达主要局限于β-珠蛋白基因，但在适应无血清生长后，观察到人类γ-珠蛋白基因和人类β-珠蛋白基因的表达相等。该细胞系进一步证明小鼠红白血病细胞的分化潜能不限于红系谱系，对于研究发育过程中珠蛋白基因调控以及双潜能红系/巨核系祖细胞终末分化的潜在机制应是有用的。
