Akiyama H, Uraroongroj M, Miyahara M, Goda Y, Toyoda M
National Institute of Health Sciences, Tokyo, Japan.
Mycopathologia. 1997;140(3):157-61. doi: 10.1023/a:1006818226376.
Fumonisins B1 (FB1) and B2 (FB2) were isocratically separated on a fluorocarbon column without using an ion pair reagent and nonvolatile buffer during the HPLC and were detected by an o-phthalaldehyde postcolumn derivatization system using a fluorescence detector. The minimum detectable concentrations of FB1 and FB2 in corn by this system were 0.01 micrograms/g and 0.01 micrograms/g, respectively. The separated fumonisins were further identified by a directly interfaced ion trap MS using electrospray ionization. FB1 and FB2 in naturally contaminated corn were identified in the selective ion monitoring mode at concentrations of 3.75 micrograms/g and 1.44 micrograms/g, respectively.
伏马毒素B1（FB1）和B2（FB2）在高效液相色谱（HPLC）分析过程中，于不含离子对试剂和非挥发性缓冲剂的碳氟化合物柱上进行等度分离，并通过邻苯二甲醛柱后衍生化系统结合荧光检测器进行检测。该系统对玉米中FB1和FB2的最低检测浓度分别为0.01微克/克和0.01微克/克。分离出的伏马毒素通过直接连接的电喷雾电离离子阱质谱进一步鉴定。在自然污染玉米中，以选择性离子监测模式鉴定出FB1和FB2的浓度分别为3.75微克/克和1.44微克/克。
