Jacobs B L, Langland J O, Brandt T
Department of Microbiology and the Graduate Program in Molecular and Cellular Biology, Arizona State University, Tempe, Arizona, 85287-2701, USA.
Methods. 1998 Jul;15(3):225-32. doi: 10.1006/meth.1998.0626.
Several viruses have been shown to code for proteins that specifically bind to double-stranded RNA or RNA with large amounts of secondary structure. These proteins have been implicated in providing interferon resistance to viruses and in inhibiting induction of apoptosis by viruses, and have been suggested to be involved in regulation of viral and cellular protein synthesis in infected cells. This article describes methods for detecting and analyzing viral double-stranded RNA-binding proteins.
已有研究表明，几种病毒可编码特异性结合双链RNA或具有大量二级结构的RNA的蛋白质。这些蛋白质与病毒的干扰素抗性以及抑制病毒诱导的细胞凋亡有关，并且有人认为它们参与了感染细胞中病毒和细胞蛋白质合成的调控。本文介绍了检测和分析病毒双链RNA结合蛋白的方法。
