Poli A, Mancianti F, Carli M A, Stroscio M C, Kramer L
Department of Animal Pathology, School of Veterinary Medicine, University of Pisa, Italy.
Vet Parasitol. 1998 Jul 31;78(2):79-85. doi: 10.1016/s0304-4017(98)00135-6.
A cutaneous nodule associated with Neospora caninum infection was diagnosed in a 5-year-old male Bernese cattle dog from Italy. The ulcerative lesion was 2-3 cm wide located in the skin of the tarsal region. Haematological values were normal and the dog did not show any neurological abnormalities. The dermal lesion consisted of a diffuse necrotic dermatitis with a dense infiltrate of mostly neutrophils and macrophages, surrounded by a fibrous wall. Histological sections revealed numerous tachyzoites of N. caninum scattered throughout the tissue. Diagnosis was confirmed both by immunohistochemical staining and electron microscopic examination. The dog had a 1:640 IFAT titre to N. caninum. Four weeks after surgical excision new subcutaneous nodules reappeared. The cutaneous lesions resolved following 21 days of therapy with clindamycin hydrochloride. These observations demonstrate the presence of N. caninum in Italy and confirm that neosporosis should be considered in the differential diagnosis of pyogranulomatous dermatitis in dogs. Clindamycin may be an effective treatment for cutaneous neosporosis.
在一只来自意大利的5岁雄性伯恩山犬身上诊断出与犬新孢子虫感染相关的皮肤结节。溃疡性病变位于跗关节区域皮肤，宽2 - 3厘米。血液学指标正常，且该犬未表现出任何神经学异常。皮肤病变由弥漫性坏死性皮炎组成，伴有大量主要为中性粒细胞和巨噬细胞的密集浸润，周围有纤维壁。组织学切片显示犬新孢子虫的大量速殖子散在分布于整个组织中。免疫组织化学染色和电子显微镜检查均证实了诊断。该犬对犬新孢子虫的间接荧光抗体试验（IFAT）滴度为1:
640。手术切除四周后，新的皮下结节再次出现。在使用盐酸克林霉素治疗21天后，皮肤病变消退。这些观察结果证明了意大利存在犬新孢子虫，并证实犬新孢子虫病应在犬脓性肉芽肿性皮炎的鉴别诊断中予以考虑。克林霉素可能是治疗皮肤新孢子虫病的有效药物。
