Piazzolla G, Tortorella C, Fiore G, Serrone M, Caccetta L, Jirillo E, Schiraldi O, Antonaci S
Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Policlinico, Italy.
Immunopharmacol Immunotoxicol. 1998 Aug;20(3):337-54. doi: 10.3109/08923979809034818.
In the present study, intrahepatic CD8+ lymphocyte infiltrates as well as HLA class I and CD54 (ICAM-1) antigen expression at both tissue and serum levels were evaluated in 54 untreated patients with chronic hepatitis C stratified on the basis of histological diagnosis (Chronic Persistent Hepatitis/Chronic Lobular Hepatitis -CPH/CLH- and Chronic Active Hepatitis -CAH-: 22 and 32 subjects, respectively). The relationships between soluble HLA-I (sHLA-I) and ICAM-1 (sICAM-1) serum levels and their membrane-bound counterparts, CD8+ liver infiltration and serum alanine aminotransferase (ALT) were also studied. A strong HLA-I and CD54 tissue expression, associated to the presence of CD8+ cell infiltrates in necro-inflammatory areas, and elevated sHLA-I and sICAM-1 serum amounts were observed in all patients. At the same time, no difference was found at tissue level between the two groups of patients with respect to the mean scores of HLA-I and CD54 expression, while CAH subjects displayed a significantly higher CD8 periportal and lobular reactivity in comparison to the other subset. Serological assays outlined higher values of circulating HLA-I molecules in CPH/CLH patients and higher sICAM-1 levels in the CAH group. Finally, a negative correlation was found between sHLA-I and ALT in CAH subjects while, in all patients, sICAM-1 positively correlated with both CD8 tissue infiltration and ALT. Our findings confirm the occurrence of an immune activation status during chronic hepatitis C and suggest that sHLA-I molecules might play a down-modulating role on immunoresponsiveness of these patients.
在本研究中，对54例未经治疗的慢性丙型肝炎患者进行了评估，这些患者根据组织学诊断进行分层（慢性持续性肝炎/慢性小叶性肝炎-CPH/CLH-和慢性活动性肝炎-CAH-：分别为22例和32例），评估了肝内CD8+淋巴细胞浸润以及组织和血清水平上的HLA I类和CD54（细胞间黏附分子-1）抗原表达。还研究了可溶性HLA-I（sHLA-I）和ICAM-1（sICAM-1）血清水平与其膜结合对应物、CD8+肝浸润和血清丙氨酸氨基转移酶（ALT）之间的关系。在所有患者中均观察到HLA-I和CD54组织的强表达，与坏死性炎症区域中CD8+细胞浸润的存在相关，以及sHLA-I和sICAM-1血清量升高。同时，两组患者在组织水平上HLA-I和CD54表达的平均评分没有差异，而与另一亚组相比，CAH患者的CD8门静脉周围和小叶反应性显著更高。血清学检测显示CPH/CLH患者循环HLA-I分子的值更高，CAH组的sICAM-1水平更高。最后，在CAH患者中发现sHLA-I与ALT之间呈负相关，而在所有患者中，sICAM-1与CD8组织浸润和ALT均呈正相关。我们的研究结果证实了慢性丙型肝炎期间免疫激活状态的存在，并表明sHLA-I分子可能对这些患者的免疫反应性起下调作用。
