检测方法对血清中头孢曲松游离浓度测定的影响。
Influence of assay methodology on the measurement of free serum ceftriaxone concentrations.
作者信息
Kohlhepp S J, Gilbert D N, Leggett J E
机构信息
Earle A. Chiles Research Institute, Providence Portland Medical Center, Portland, Oregon, USA.
出版信息
Antimicrob Agents Chemother. 1998 Sep;42(9):2259-61. doi: 10.1128/AAC.42.9.2259.
The influence of assay methodology on the measurement of the active free fraction of ceftriaxone in plasma was determined. The free fraction was measured by three methods: agar diffusion bioassay, precipitation of plasma protein with methanol followed by high-performance liquid chromatography (HPLC) of the supernatant, and ultrafiltration of plasma followed by HPLC of the filtrate. In human serum, the free ceftriaxone levels were significantly lower (P = 0.03) when measured on ultrafiltrates compared to the other two methods. This difference disappeared when dolphin serum was studied. After ultrafiltration, human serum was shown, by Scatchard plot analysis, to have two ceftriaxone binding sites. Species differences were also demonstrated. Hence, in humans, determination of free plasma ceftriaxone varies with the assay method employed.
测定了分析方法对血浆中头孢曲松活性游离分数测定的影响。游离分数通过三种方法进行测定：琼脂扩散生物测定法、用甲醇沉淀血浆蛋白然后对上清液进行高效液相色谱法（HPLC），以及对血浆进行超滤然后对滤液进行HPLC。在人血清中，与其他两种方法相比，通过超滤法测定时游离头孢曲松水平显著更低（P = 0.03）。在研究海豚血清时这种差异消失了。通过Scatchard图分析表明，超滤后人血清有两个头孢曲松结合位点。还证明了种属差异。因此，在人类中，血浆中游离头孢曲松的测定因所采用的分析方法而异。
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