Valtuille R, Fernández J L, Berridi J, Moretto H, del Pino N, Rendo P, Lef L
Terapia Sustitutiva Renal, Monte Grande, Buenos Aires, and Bio Sidus, Buenos Aires, Argentina.
Nephron. 1998 Oct;80(2):194-6. doi: 10.1159/000045166.
The passage of hepatitis C virus (HCV) across the dialysis membrane is a controversial issue. We performed a study applying extreme conditions of permeability to the dialysis membrane and avoiding the use of heparin and dialysis bath that might interfere with polymerase chain reaction (PCR) results. We obtained samples from the ultrafiltrate at the beginning of 18 hemodialysis sessions carried out in 6 HCV RNA-positive patients. HCV RNA was detected by PCR in 3 (16.7%) ultrafiltrate samples belonging to 1 of the patients. HCV genotype was the same as that found in positive ultrafiltrate samples and in the serum corresponding to this patient. The viral load of this patient was under the levels detectable by the assay employed. Therefore, contamination of the ultrafiltrate may constitute a potential risk for HCV transmission in hemodialysis units.
丙型肝炎病毒（HCV）通过透析膜是一个存在争议的问题。我们进行了一项研究，对透析膜施加极端的渗透条件，并避免使用可能干扰聚合酶链反应（PCR）结果的肝素和透析液。我们从6例HCV RNA阳性患者进行的18次血液透析治疗开始时的超滤物中获取样本。通过PCR在属于1例患者的3份（16.7%）超滤物样本中检测到HCV RNA。HCV基因型与在阳性超滤物样本以及该患者相应血清中发现的基因型相同。该患者的病毒载量低于所采用检测方法的可检测水平。因此，超滤物污染可能构成血液透析单位中HCV传播的潜在风险。
