Nakamura Y, Yamamoto M, Itoh S, Haratake A, Nakano Y, Hashimoto T
Department of Pathology, St. Mary's Hospital, Kurume University School of Medicine, Japan.
J Neuropathol Exp Neurol. 1998 Sep;57(9):858-65. doi: 10.1097/00005072-199809000-00007.
We examined the expression of selected growth factors, growth factor receptors, elements of extracellular matrix and cell adhesion molecules in the germinal matrix layer (GML) utilizing immunohistochemistry and reverse transcriptase polymerase chain reaction. At autopsy brain samples from 10 neonatal infants were used. Epidermal growth factor receptor (EGFR) was significantly expressed in the matrix cells. While transforming growth factor alpha and heparin-binding epidermal growth factor-like growth factor were found in the matrix cells or vascular wall as ligands, epidermal growth factor was not expressed. EGFR and its ligands are thought to be important factors for the maintenance of the matrix cells and cell-to-cell interactions. Insulin like growth factor I, its receptor Ibeta and tenascin were found in the stroma of the GML and periventricular region. Vascular endothelial growth factor and receptor Flk-1, laminin A and B2, fibronectin, collagen type IV and integrins such as beta3, alpha5beta1 and alphaVbeta3 were found mainly in or around the vascular wall indicating their important roles for vascularization. Transforming growth factor beta2 and its receptor II were expressed in the matrix cells and/or vascular wall suggesting a role in proliferation and/or regression of the vasculature. CD44 and Thy-1 were also expressed in the matrix cells.
我们利用免疫组织化学和逆转录聚合酶链反应，检测了生发基质层（GML）中特定生长因子、生长因子受体、细胞外基质成分和细胞黏附分子的表达。尸检时使用了10例新生儿的脑样本。表皮生长因子受体（EGFR）在基质细胞中显著表达。虽然在基质细胞或血管壁中发现转化生长因子α和肝素结合表皮生长因子样生长因子作为配体，但未检测到表皮生长因子的表达。EGFR及其配体被认为是维持基质细胞和细胞间相互作用的重要因素。胰岛素样生长因子I、其受体Iβ和腱生蛋白在GML的基质和脑室周围区域被发现。血管内皮生长因子及其受体Flk-1、层粘连蛋白A和B2、纤连蛋白、IV型胶原以及整合素如β3、α5β1和αVβ3主要在血管壁内或其周围被发现，表明它们在血管形成中起重要作用。转化生长因子β2及其受体II在基质细胞和/或血管壁中表达，提示其在血管增殖和/或消退中起作用。CD44和Thy-1也在基质细胞中表达。
