基于聚合酶链反应的免疫功能正常肺结核患者血液中结核分枝杆菌的快速检测
PCR-based rapid detection of Mycobacterium tuberculosis in blood from immunocompetent patients with pulmonary tuberculosis.
作者信息
Ahmed N, Mohanty A K, Mukhopadhyay U, Batish V K, Grover S
机构信息
Molecular Biology Unit, National Dairy Research Institute, Karnal 132001, India.
出版信息
J Clin Microbiol. 1998 Oct;36(10):3094-5. doi: 10.1128/JCM.36.10.3094-3095.1998.
Abstract
A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.
摘要
开发了一种基于插入序列IS1081的聚合酶链反应（PCR）检测方法，用于检测外周血中的结核分枝杆菌复合群微生物。该方法应用于患有局限性肺结核的免疫功能正常个体的血样。16份血样中有7份（43.75%）的结核分枝杆菌复合群循环DNA拷贝呈阳性。
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