Cantürk Z, Cantürk N Z, Onen F
Cumhuriyet University, School of Medicine, Department of Internal Medicine, Sivas, Turkey.
Endocr Res. 1998 May;24(2):141-57. doi: 10.1080/07435809809135524.
Diabetic patients are more prone to infection and evidence for an immunologic defect superimposed upon the metabolic abnormalities of diabetes is convincing. Neutrophils play a critical role in the host defense mechanism against various bacterial infections, and it is suggested that impaired neutrophil functions cause susceptibility to infections in diabetic patients. To explore the possibility that Granulocyte colony-stimulating factor (G-CSF) may be useful to prevent the morbidity and mortality caused by infections in diabetics. We studied the effect of G-CSF against septicemia in diabetic rats. Forty eight rats were divided into seven equal groups. The IInd, IVth-VIth groups were made diabetic by single intraperitoneal injection of streptozotocin. Fourth, VIth and VIIth groups were made septicemic by cecal ligation and perforation at the end of the second week of streptozotocin injection. G-CSF was subcutaneously injected into IIIrd, Vth and VIth groups. White blood cell count, neutrophil counts and function were determined. Rats in all groups were also observed for seven days for survival. White blood cells, neutrophil and lymphocyte counts and the neutrophil phagocytosis index decreased but neutrophil adherence rate was not different in diabetic group II (p<0.05). All these variables were significantly diminished in diabetes and sepsis-induced group IV (p<0.05). G-CSF injections improved all variables except neutrophil adherence. Cumulative survival ratio was better in G-CSF-injected group VI than in ceftriaxon-administrated group VII (p<0.05). In conclusion, G-CSF increased neutrophil counts, developed neutrophil functions and improved survival. These results suggest that G-CSF may be useful as a drug to prevent bacterial infection in diabetic patients.
糖尿病患者更容易感染，而且有令人信服的证据表明，糖尿病代谢异常之上叠加了免疫缺陷。中性粒细胞在宿主抵御各种细菌感染的防御机制中起关键作用，有人认为中性粒细胞功能受损会导致糖尿病患者易受感染。为了探讨粒细胞集落刺激因子（G-CSF）是否可能有助于预防糖尿病患者因感染导致的发病和死亡。我们研究了G-CSF对糖尿病大鼠败血症的影响。48只大鼠被分成7个相等的组。第二、四至六组通过单次腹腔注射链脲佐菌素制成糖尿病模型。在链脲佐菌素注射第二周结束时，第四、六和七组通过盲肠结扎和穿孔制成败血症模型。G-CSF皮下注射到第三、五和六组。测定白细胞计数、中性粒细胞计数和功能。所有组的大鼠也观察7天的生存情况。糖尿病第二组白细胞、中性粒细胞和淋巴细胞计数以及中性粒细胞吞噬指数下降，但中性粒细胞黏附率无差异（p<0.05）。在糖尿病和败血症诱导的第四组中，所有这些变量均显著降低（p<0.05）。G-CSF注射改善了除中性粒细胞黏附外的所有变量。G-CSF注射的第六组累积生存率优于头孢曲松给药的第七组（p<0.05）。总之，G-CSF增加了中性粒细胞计数，改善了中性粒细胞功能并提高了生存率。这些结果表明，G-CSF可能作为一种药物用于预防糖尿病患者的细菌感染。
