Ishigami A, Kuramoto M, Yamada M, Watanabe K, Senshu T
Department of Cell Chemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
FEBS Lett. 1998 Aug 14;433(1-2):113-8. doi: 10.1016/s0014-5793(98)00893-x.
Peptidylarginine deiminases (PADs) are a group of enzymes which convert protein arginine residues to citrulline residues. Using rat muscle PAD cDNA as a probe, we obtained two novel cDNAs, PAD-R11 and PAD-R4, from immortalized rat keratinocytes treated with all-trans retinoic acid. Comparison of the deduced amino acid sequences with those of muscle and hair follicle enzymes showed high conservation in the C-terminal region. Recombinant proteins encoded by both PAD-R11 and PAD-R4 showed the enzyme activities. That of PAD-R11 showed a characteristic feature of the enzyme found in the epidermis.
