• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Acylation of Streptomyces type II polyketide synthase acyl carrier proteins.

作者信息

Crosby J, Byrom K J, Hitchman T S, Cox R J, Crump M P, Findlow I S, Bibb M J, Simpson T J

机构信息

School of Chemistry, University of Bristol, Cantock's Close, UK.

出版信息

FEBS Lett. 1998 Aug 14;433(1-2):132-8. doi: 10.1016/s0014-5793(98)00840-0.

DOI:10.1016/s0014-5793(98)00840-0
PMID:9738947
Abstract

Acyl derivatives of type II PKS ACPs are required for in vitro studies of polyketide biosynthesis. The presence of an exposed cysteine residue prevented specific chemical acylation of the phosphopantetheine thiol of the actinorhodin PKS holo ACP. Acylation studies were further complicated by intramolecular disulphide formation between cysteine 17 and the phosphopantetheine. The presence of this intramolecular disulphide was confirmed by tryptic digestion of the ACP followed by ESMS analysis of the fragments. An act Cys17Ser ACP was engineered by site-directed mutagenesis. S-Acyl adducts of act C17S, oxytetracycline and griseusin holo ACPs were rapidly formed by reaction with hexanoyl, 5-ketohexanoyl and protected acetoacetyl imidazolides. Comparisons with type 11 FAS ACPs were made.

摘要

相似文献

1
Acylation of Streptomyces type II polyketide synthase acyl carrier proteins.
FEBS Lett. 1998 Aug 14;433(1-2):132-8. doi: 10.1016/s0014-5793(98)00840-0.
2
Ability of Streptomyces spp. acyl carrier proteins and coenzyme A analogs to serve as substrates in vitro for E. coli holo-ACP synthase.链霉菌属酰基载体蛋白和辅酶A类似物在体外作为大肠杆菌全酶型酰基载体蛋白合成酶底物的能力。
Chem Biol. 1997 Jan;4(1):17-24. doi: 10.1016/s1074-5521(97)90233-7.
3
Catalytic self-acylation of type II polyketide synthase acyl carrier proteins.II型聚酮合酶酰基载体蛋白的催化自酰化作用。
Chem Biol. 1998 Jan;5(1):35-47. doi: 10.1016/s1074-5521(98)90085-0.
4
Post-translational modification of heterologously expressed Streptomyces type II polyketide synthase acyl carrier proteins.异源表达的链霉菌II型聚酮合酶酰基载体蛋白的翻译后修饰
FEBS Lett. 1997 Apr 1;405(3):267-72. doi: 10.1016/s0014-5793(97)00202-0.
5
Heterologously expressed acyl carrier protein domain of rat fatty acid synthase functions in Escherichia coli fatty acid synthase and Streptomyces coelicolor polyketide synthase systems.大鼠脂肪酸合酶的异源表达酰基载体蛋白结构域在大肠杆菌脂肪酸合酶和天蓝色链霉菌聚酮合酶系统中发挥作用。
Chem Biol. 1998 Mar;5(3):135-46. doi: 10.1016/s1074-5521(98)90058-8.
6
Polyketide synthase acyl carrier proteins from Streptomyces: expression in Escherichia coli, purification and partial characterisation.
Biochim Biophys Acta. 1995 Aug 16;1251(1):32-42. doi: 10.1016/0167-4838(95)00053-w.
7
Enzymes involved in fatty acid and polyketide biosynthesis in Streptomyces glaucescens: role of FabH and FabD and their acyl carrier protein specificity.参与浅绿链霉菌脂肪酸和聚酮化合物生物合成的酶：FabH和FabD的作用及其酰基载体蛋白特异性
Biochemistry. 2002 Aug 20;41(33):10462-71. doi: 10.1021/bi0258804.
8
Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase.耶尔森菌素合成酶HMWP1亚基的聚酮合酶和非核糖体肽基合成酶模块中载体蛋白结构域的纯化、引发及催化酰化作用
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):99-104. doi: 10.1073/pnas.98.1.99.
9
Utilization of enzymatically phosphopantetheinylated acyl carrier proteins and acetyl-acyl carrier proteins by the actinorhodin polyketide synthase.放线紫红素聚酮合酶对酶促磷酸泛酰巯基乙胺化酰基载体蛋白和乙酰化酰基载体蛋白的利用
Biochemistry. 1997 Sep 30;36(39):11757-61. doi: 10.1021/bi971350+.
10
Relationships between fatty acid and polyketide synthases from Streptomyces coelicolor A3(2): characterization of the fatty acid synthase acyl carrier protein.天蓝色链霉菌A3(2)中脂肪酸合酶与聚酮合酶的关系：脂肪酸合酶酰基载体蛋白的特性
J Bacteriol. 1996 Oct;178(19):5660-7. doi: 10.1128/jb.178.19.5660-5667.1996.

引用本文的文献

1
Deciphering the Binding Interactions between ACP and β-ketoacyl ACP Synthase III to Improve Antibiotic Targeting Using NMR Spectroscopy.解析 ACP 与β-酮酰基 ACP 合酶 III 之间的结合相互作用，利用 NMR 光谱提高抗生素靶向性。
Int J Mol Sci. 2021 Mar 24;22(7):3317. doi: 10.3390/ijms22073317.
2
Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways.探究酰基载体蛋白的结构与功能，解锁 II 型聚酮化合物生物合成途径的战略重设计。
J Biol Chem. 2021 Jan-Jun;296:100328. doi: 10.1016/j.jbc.2021.100328. Epub 2021 Jan 23.
3
Total synthesis of griseusins and elucidation of the griseusin mechanism of action.
灰黄霉素的全合成及灰黄霉素作用机制的阐明。
Chem Sci. 2019 Jun 27;10(32):7641-7648. doi: 10.1039/c9sc02289a. eCollection 2019 Aug 28.
4
Biochemical characterization of the minimal domains of an iterative eukaryotic polyketide synthase.一种迭代真核聚酮合酶最小结构域的生化特性。
FEBS J. 2018 Dec;285(23):4494-4511. doi: 10.1111/febs.14675. Epub 2018 Oct 25.
5
Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein.II型酰基载体蛋白对延伸线性和环化聚酮类似物的识别。
Chem Sci. 2016 Mar 1;7(3):1779-1785. doi: 10.1039/c5sc03864b. Epub 2015 Dec 10.
6
Iterative assembly of two separate polyketide chains by the same single-module bacterial polyketide synthase in the biosynthesis of HSAF.在哈茨木霉菌素生物合成过程中，同一单模块细菌聚酮合酶对两条独立的聚酮链进行迭代组装。
Angew Chem Int Ed Engl. 2014 Jul 14;53(29):7524-7530. doi: 10.1002/anie.201403500. Epub 2014 May 30.
7
Sulfonyl 3-alkynyl pantetheinamides as mechanism-based cross-linkers of acyl carrier protein dehydratase.磺酰基 3-炔基 pantetheinamides 作为酰基辅酶 A 脱水酶的基于机制的交联剂。
J Am Chem Soc. 2013 Jun 19;135(24):8846-9. doi: 10.1021/ja4042059. Epub 2013 Jun 4.
8
Binding and "pKa" modulation of a polycyclic substrate analogue in a type II polyketide acyl carrier protein.在 II 型聚酮类酰基载体蛋白中，多环底物类似物的结合和“pKa”调节。
ACS Chem Biol. 2011 May 20;6(5):413-8. doi: 10.1021/cb200004k. Epub 2011 Feb 22.