Robinson C, Hynds P J, Robinson D, Mant A
Department of Biological Sciences, University of Warwick, Coventry, UK.
Plant Mol Biol. 1998 Sep;38(1-2):209-21.
The assembly of the photosynthetic apparatus requires the import of numerous cytosolically synthesised proteins and their correct targeting into or across the thylakoid membrane. Biochemical and genetic studies have revealed the operation of several targeting pathways for these proteins, some of which are used for thylakoid lumen proteins whereas others are utilised by membrane proteins. Some pathways can be traced back to the prokaryotic ancestors of chloroplasts but at least one pathway appears to have arisen in response to the transfer of genes from the organelle to the nucleus. In this article we review recent findings in this field that point to the operation of a mechanistically unique protein translocase in both plastids and bacteria, and we discuss emerging data that reconcile the remarkable variety of targeting pathways with the natures of the substrate precursor proteins.
光合装置的组装需要导入众多在细胞质中合成的蛋白质，并将它们正确靶向到类囊体膜内或穿过类囊体膜。生化和遗传学研究揭示了这些蛋白质的几种靶向途径，其中一些用于类囊体腔蛋白，而另一些则被膜蛋白所利用。一些途径可以追溯到叶绿体的原核祖先，但至少有一种途径似乎是为了应对基因从细胞器转移到细胞核而产生的。在本文中，我们综述了该领域的最新发现，这些发现表明在质体和细菌中存在一种机制独特的蛋白质转运体，并且我们讨论了新出现的数据，这些数据将靶向途径的显著多样性与底物前体蛋白的性质协调起来。
