Kim E M, Welch C C, Grace M K, Billington C J, Levine A S
Minnesota Obesity Center and Research Service (151), VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA.
Brain Res. 1998 Sep 21;806(1):117-21. doi: 10.1016/s0006-8993(98)00755-0.
This study examined the effect of feeding either a bland cornstarch-based diet (BCD) or a highly palatable, high fat diet containing sucrose (HPD) on hypothalamic arcuate nucleus (ARC) gene expression for neuropeptide-Y (NPY). Male Sprague-Dawley rats received either BCD ad libitum, HPD ad libitum, HPD pair-fed to the caloric intake of the BCD, or the HPD at 60% of ad libitum HPD intake for 7 days. Animals receiving the HPD ad libitum consumed more calories and gained more weight than animals receiving the BCD (P<0.001). The HPD did not affect ARC NPY mRNA levels, whether the subjects were allowed to overeat or pair-fed to the BCD (P>0.05). However, feeding the HPD at 60% of ad libitum intake of the HPD, increased NPY mRNA levels in the ARC relative to the other treatments (P<0.01). The present data are consistent with the view that NPY in ARC responds to energy deficits rather than to hyperphagia stimuli related to palatability.
本研究检测了喂食平淡的玉米淀粉基饮食（BCD）或含有蔗糖的高度可口的高脂肪饮食（HPD）对下丘脑弓状核（ARC）中神经肽Y（NPY）基因表达的影响。雄性斯普拉格-道利大鼠自由摄取BCD、自由摄取HPD、将HPD按BCD的热量摄入量进行配对喂食，或将HPD按自由摄取量的60%喂食7天。自由摄取HPD的动物比摄取BCD的动物消耗更多热量且体重增加更多（P<0.001）。无论受试者是被允许暴饮暴食还是与BCD进行配对喂食，HPD均不影响ARC中NPY的mRNA水平（P>0.05）。然而，将HPD按自由摄取量的60%喂食，相对于其他处理，ARC中NPY的mRNA水平升高（P<0.01）。目前的数据与以下观点一致，即ARC中的NPY对能量不足作出反应，而非对与适口性相关的摄食亢进刺激作出反应。
