Chen Y C, Chen Q S, Lei J L, Wang S L
Department of Anatomy, Beijing Medical University, Beijing 100083, China.
Brain Res. 1998 Sep 28;806(2):238-45. doi: 10.1016/s0006-8993(98)00770-7.
We investigated the effect of a moderate amount of prolonged physical training initiated at 3 months of age on the expression of GAP-43 and synaptophysin in the hippocampal formation. C57BL/6J mice were divided into three groups which were trained (24 months old), sedentary (24 months old) and young (3 months old). From 3 months of age on, mice of trained group were treated with voluntary running wheel for 1 h each day (5 days per week) until 24 months of age (21 months running), whereas mice of sedentary group were put in immobilized wheels for the same time. Using immunohistochemistry and image analysis system, GAP-43 and synaptophysin were analysed quantitatively in the CA1, CA3 areas and the dentate gyrus of the hippocampal formation. As compared with young mice, the densities of GAP-43 and synaptophysin immunostaining showed a significant decrease in the hippocampal formation in sedentary group (P<0.01). After 21 months of running, the densities of GAP-43 and synaptophysin immunostaining significantly increased in the examined areas of the hippocampal formation in trained mice compared to their age-matched sedentary controls (P<0.05, 0.01). These results indicate that a moderate amount of prolonged physical training could modify the age-related decrease of the expression of GAP-43 and synaptophysin in the hippocampal formation, and that the increased expression of GAP-43 and synaptophysin might be associated with the anatomical sprouting and synaptogenesis.
我们研究了3月龄开始的适量长期体育锻炼对海马结构中生长相关蛋白43（GAP - 43）和突触素表达的影响。将C57BL/6J小鼠分为三组，即训练组（24月龄）、久坐组（24月龄）和幼龄组（3月龄）。从3月龄起，训练组小鼠每天在活动跑轮上运动1小时（每周5天），直至24月龄（共运动21个月），而久坐组小鼠则置于固定跑轮相同时间。采用免疫组织化学和图像分析系统，对海马结构的CA1、CA3区及齿状回中GAP - 43和突触素进行定量分析。与幼龄小鼠相比，久坐组小鼠海马结构中GAP - 43和突触素免疫染色密度显著降低（P<0.01）。经过21个月的跑步训练后，与年龄匹配的久坐对照组相比，训练组小鼠海马结构各检测区域中GAP - 43和突触素免疫染色密度显著增加（P<0.05，0.01）。这些结果表明，适量的长期体育锻炼可改变海马结构中与年龄相关的GAP - 43和突触素表达下降，且GAP - 43和突触素表达的增加可能与解剖性发芽和突触形成有关。
