Wagner S N, Wagner C, Reinhold U, Funk R, Zöller M, Goos M
Department of Dermatology, Medical School, University of Essen, Germany.
J Invest Dermatol. 1998 Sep;111(3):464-71. doi: 10.1046/j.1523-1747.1998.00302.x.
The remarkable functional diversity of the cell surface receptor CD44 may be due to expression of multiple variant isoforms generated by alternative splicing of variant exons. Functional and correlative data implicate a role of CD44 variant isoforms in adhesion dependent processes such as lymphocyte recirculation and tumor progression and metastasis. We have analyzed 25 primary cutaneous lymphomas and 35 reactive lymphoid cell skin infiltrates or T cell-mediated skin diseases for the expression of CD44 variant isoforms. Irrespective of histologic typing, staging, and grading, cutaneous lymphomas as well as nonmalignant skin-infiltrating CD3+ CD4+ and CD8+ T and CD19+ B lymphocytes exhibited a strong expression of CD44v10 and a moderate expression of CD44v3 as determined by immunohistochemistry, immunofluorescence microscopy, and mRNA analysis. Expression of v5, v6, v7, and v9-containing CD44 variant isoforms was not detected. Furthermore, flow cytometry revealed expression of CD44v10 on a significant proportion of peripheral blood lymphocytes from Sézary's syndrome patients and a remarkable co-expression with cutaneous lymphocyte antigen. These results indicate a distinct CD44 variant isoform expression pattern associated with skin-homing lymphocytes different to lymphatic cells at noncutaneous sites. This differential expression pattern of CD44 variant isoforms may contribute to the development of lymphocyte skin infiltrates and/or the unique biologic behavior of cutaneous lymphomas.
细胞表面受体CD44显著的功能多样性可能归因于可变外显子选择性剪接产生的多种变异亚型的表达。功能及相关数据表明，CD44变异亚型在诸如淋巴细胞再循环、肿瘤进展和转移等黏附依赖性过程中发挥作用。我们分析了25例原发性皮肤淋巴瘤以及35例反应性淋巴样细胞皮肤浸润或T细胞介导的皮肤疾病中CD44变异亚型的表达情况。通过免疫组织化学、免疫荧光显微镜检查及mRNA分析确定，无论组织学类型、分期和分级如何，皮肤淋巴瘤以及非恶性皮肤浸润的CD3⁺ CD4⁺和CD8⁺ T淋巴细胞及CD19⁺ B淋巴细胞均强烈表达CD44v10，中度表达CD44v3。未检测到含v5、v6、v7和v9的CD44变异亚型的表达。此外，流式细胞术显示， Sézary综合征患者外周血淋巴细胞中有相当一部分表达CD44v10，且与皮肤淋巴细胞抗原显著共表达。这些结果表明，与非皮肤部位的淋巴细胞不同，与皮肤归巢淋巴细胞相关的CD44变异亚型表达模式具有独特性。CD44变异亚型的这种差异表达模式可能有助于淋巴细胞皮肤浸润的发展和/或皮肤淋巴瘤独特的生物学行为。
