Dabiri S, Hayes M M, Meymandi S S, Basiri M, Soleimani F, Mousavi M R
Pathology Department, Kerman Medical School, Iran.
Diagn Cytopathol. 1998 Sep;19(3):182-5. doi: 10.1002/(sici)1097-0339(199809)19:3<182::aid-dc5>3.0.co;2-f.
Exfoliative cytology smears from the lesions of 179 patients with cutaneous leishmaniasis due to Leishmania tropica were studied with specific reference to cellular reactions and their effect on the parasite. Aggregates of the parasite (so-called Leishmania Donovan bodies) were present within macrophages and in some fibroblasts. The nature of the inflammatory reaction to the disease was studied by performing differential counts of the inflammatory cells present in the smears. These were correlated with the number of Leishman Donovan bodies. There was an inverse relationship between the number of Leishman Donovan bodies and the percentage of small lymphocytes, neutrophils, and type I macrophages. It is postulated that aggregates of activated macrophages (designated types II and III) and the Leishmanian milieu (sticky matrix) protect the amastigote Leishmania parasites from being eradicated by the inflammatory and immune reaction. The cytoplasmic blebbing of the parasitophorous vacuoles and cell to cell connection of the activated histiocytes could be shown by the CD-68 immunostaining of the tissue biopsy.
对179例热带利什曼原虫引起的皮肤利什曼病患者病变部位的脱落细胞学涂片进行了研究，特别关注细胞反应及其对寄生虫的影响。巨噬细胞和一些成纤维细胞内存在寄生虫聚集体（所谓的杜氏利什曼原虫小体）。通过对涂片中存在的炎性细胞进行分类计数来研究对该病的炎症反应性质。这些与杜氏利什曼原虫小体的数量相关。杜氏利什曼原虫小体的数量与小淋巴细胞、中性粒细胞和I型巨噬细胞的百分比之间存在负相关关系。据推测，活化巨噬细胞聚集体（称为II型和III型）和利什曼原虫微环境（粘性基质）可保护无鞭毛体利什曼原虫免受炎症和免疫反应的根除。通过组织活检的CD-68免疫染色可显示寄生泡的细胞质泡化和活化组织细胞的细胞间连接。
