乳脂肪球膜蛋白的鉴定。I. 糖蛋白B的分离及部分特性分析

Identification of the milk fat globule membrane proteins. I. Isolation and partial characterization of glycoprotein B.

作者信息

Basch J J, Farrell H M, Greenberg R

出版信息

Biochim Biophys Acta. 1976 Nov 2;448(4):589-98. doi: 10.1016/0005-2736(76)90112-7.

Abstract

The salt soluble proteins from the fat globule membrane of cow's milk were resolved into three fractions by Sephadex column chromatography in sodium dodecyl sulfate. One of the fractions, termed glycoprotein B, was purified by rechromatography to essentially one band on sodium dodecyl sulfate gel electrophoresis. It was found to contain 14% carbohydrate including sialic acid, mannose, galactose, glucose, glucosamine and galactosamine. The amino acid composition of glycoprotein B was determined; it has amino terminal serine and carboxyl terminal leucine. The molecular weight of this glycoprotein as estimated by sodium dodecyl sulfate gel electrophoresis is 49 500.

摘要

牛奶脂肪球膜中的盐溶性蛋白质通过十二烷基硫酸钠存在下的葡聚糖凝胶柱色谱法被分离成三个组分。其中一个组分称为糖蛋白B，通过再色谱法纯化，在十二烷基硫酸钠凝胶电泳上基本呈现为一条带。发现它含有14%的碳水化合物，包括唾液酸、甘露糖、半乳糖、葡萄糖、氨基葡萄糖和半乳糖胺。测定了糖蛋白B的氨基酸组成；它的氨基末端是丝氨酸，羧基末端是亮氨酸。通过十二烷基硫酸钠凝胶电泳估计该糖蛋白的分子量为49500。