Park Y K, Chi S G, Park H R, Yang M H, Unni K K
Department of Pathology, College of Medicine, Kyung Hee University, Seoul, Korea.
J Korean Med Sci. 1998 Aug;13(4):395-9. doi: 10.3346/jkms.1998.13.4.395.
Ewing's sarcoma is a poorly characterized malignant tumor with a relatively uniform histologic appearance, made up of densely packed small cells with round to oval nuclei, without distinct cell borders and without any structural differentiation. Often the diagnosis has to be made by exclusion. Recently, it has been made possible to identify characteristic chromosomal rearrangements associated with certain solid tumors. More than 85% of Ewing's sarcoma and peripheral neuroectodermal tumor present a specific t(11;22)(q24;q12) balanced translocation, resulting in the production of a novel chimerical EWS/FLI-1 message. Using oligonucleotide primers derived from EWS and FLI-1 complementary DNAs, we were able to use reverse transcription-polymerase chain reaction (RT-PCR) as a diagnostic tool. The described nested RT-PCR method as another supportive diagnostic method enables pathologists to differentiate small blue cell tumors not only to make correct diagnosis but also to investigate retrospective archival tumor samples, using formalin fixed paraffin embedded tissue as a source of RNA.
尤因肉瘤是一种特征描述较少的恶性肿瘤，组织学表现相对一致，由紧密排列的小细胞组成，细胞核呈圆形至椭圆形，无明显细胞边界且无任何结构分化。通常必须通过排除法来做出诊断。最近，已经能够识别与某些实体瘤相关的特征性染色体重排。超过85%的尤因肉瘤和外周神经外胚层肿瘤呈现特定的t(11;22)(q24;q12)平衡易位，导致产生一种新的嵌合EWS/FLI-1信息。使用源自EWS和FLI-1互补DNA的寡核苷酸引物，我们能够将逆转录-聚合酶链反应（RT-PCR）用作诊断工具。所描述的巢式RT-PCR方法作为另一种辅助诊断方法，使病理学家能够区分小蓝细胞肿瘤，不仅能做出正确诊断，还能使用福尔马林固定石蜡包埋组织作为RNA来源，对回顾性存档肿瘤样本进行研究。
