Hyde G
Faculty of Life Sciences, University of New South Wales, Sydney, 2052, Australia.
Fungal Genet Biol. 1998 Jun-Jul;24(1-2):14-23. doi: 10.1006/fgbi.1998.1068.
This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development are highly dependent upon Ca2+. The methodologies for imaging Ca2+ fluxes continue to develop rapidly. These methodologies are explained here in a style that should be accessible to a newcomer to the field, hopefully forming a bridge to the more complex methodological literature.
本文旨在鼓励更多真菌生物学家考虑对细胞质Ca2+通量进行成像研究。与其他生物相比，针对真菌，尽管其他研究方法表明真菌的生长和发育高度依赖Ca2+，但对Ca2+通量在信号转导和一般细胞活动中的作用进行表征的尝试却非常少。用于成像Ca2+通量的方法正在迅速发展。本文以该领域新手能够理解的方式对这些方法进行解释，希望能为通向更复杂的方法学文献搭建一座桥梁。
