原位观察大鼠心肌中的脱氧肌红蛋白和血红蛋白信号。

Observing the deoxy myoglobin and hemoglobin signals from rat myocardium in situ.

作者信息

Tran T K, Kreutzer U, Jue T

机构信息

Department of Biological Chemistry, University of California Davis, 95616-8635, USA.

出版信息

FEBS Lett. 1998 Sep 4;434(3):309-12. doi: 10.1016/s0014-5793(98)01001-1.

DOI:10.1016/s0014-5793(98)01001-1

PMID:9742944

Abstract

1H NMR proximal histidyl NdeltaH signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO2 is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O2, the erythrocyte Hb is partially desaturated and yields the alpha and beta proximal histidyl NdeltaH signals of deoxy Hb. The detection of the Hb signals clarifies the debate about the NMR visibility of erythrocyte Hb in vivo and augments the strategy to observe tissue pO2.

摘要

在大鼠心肌原位中，脱氧血红蛋白（Hb）和肌红蛋白（Mb）的1H NMR近端组氨酸NδH信号是可区分的。在常氧静息状态下，血液和组织的pO2足以使Mb和Hb都饱和。未检测到脱氧Mb或Hb信号。在吸入12%氧气的情况下，红细胞Hb部分去饱和，并产生脱氧Hb的α和β近端组氨酸NδH信号。Hb信号的检测澄清了关于体内红细胞Hb的NMR可见性的争论，并增强了观察组织pO2的策略。