Rujithamkul N, Majlessi L, Denoyelle C, Bordenave G
Unité d'Immunophysiologie Moléculaire, Institut Pasteur, 25, rue du Docteur-Roux, Paris Cedex 15, 75724, France.
Cell Immunol. 1998 Aug 25;188(1):41-8. doi: 10.1006/cimm.1998.1345.
The growth of IgG2ab-producing CB101 myeloma cells, subcutaneously or intraperitoneally inoculated into histocompatible BALB/c Igha mice sensitized against this Ig allotype, was delayed by 2-4 weeks compared to normal mice. While IgG2ab production was detected in the sera of 75-100% of normal mice, it was irreversibly inhibited in 100% of sensitized mice. IgG2ab suppression (IgG2ab sup) was also systematically obtained in sensitized but not normal recipients, implanted ip with a 0.1-micrometer-pore diffusion chamber (DC) containing CB101 cells. This time, the specific IgG2ab sup was reversible in vitro in the absence of anti-IgG2ab T cells. Adoptive transfer, of unfractionated or T but not B splenocytes from their sensitized counterparts into normal mice, 1 day before DC implantation, induced IgG2ab sup as well. These results indicate that, in these experimental circumstances, IgG2ab sup can also be mediated by diffusible suppressive factors produced by the effector T cells, without direct T-B-cell contact.
将产生IgG2ab的CB101骨髓瘤细胞皮下或腹腔内接种到对这种Ig同种异型敏感的组织相容性BALB/c Igha小鼠体内，与正常小鼠相比，其生长延迟了2至4周。虽然在75%至100%的正常小鼠血清中检测到了IgG2ab的产生，但在100%的致敏小鼠中其产生被不可逆地抑制。在致敏但非正常的受体小鼠中，通过腹腔内植入含有CB101细胞的0.1微米孔径的扩散室（DC），也系统性地获得了IgG2ab抑制（IgG2ab sup）。这一次，在没有抗IgG2ab T细胞的情况下，特异性IgG2ab sup在体外是可逆的。在植入DC前1天，将来自致敏小鼠的未分级脾细胞或T细胞而非B细胞过继转移到正常小鼠体内，也诱导了IgG2ab sup。这些结果表明，在这些实验条件下，IgG2ab sup也可由效应T细胞产生的可扩散抑制因子介导，而无需T细胞与B细胞直接接触。
