Fan J Y, Boyce C S, Cuff C F
Department of Microbiology and Immunology, Robert C. Byrd Health Sciences Center of West Virginia University, Morgantown, West Virginia 26506-9177, USA.
Cell Immunol. 1998 Aug 25;188(1):55-63. doi: 10.1006/cimm.1998.1350.
Enteric infection of mice with reovirus serotype 1 elicits antibody and cytotoxic T-lymphocytes in gut-associated lymphoid tissue (GALT). This led to the hypothesis that T-helper 1 (Th1) and T-helper 2 (Th2) responses develop in GALT. Reverse transcriptase-polymerase chain reactions on RNA from Peyer's patches (PP), intraepithelial lymphocytes (IEL), and lamina propria (LP) lymphocytes demonstrated that interferon (IFN)-gamma message was increased in PP and IEL, but not in LP following infection. No increase in mRNA for interleukin (IL)-4, IL-5, or IL-6 was detected. IFN-gamma, IL-5, and IL-6 were produced in in vitro cultures of PP 4-10 days postinfection. PP and spleen lymphocytes from infected mice produced IFN-gamma, but no IL-5 following in vitro restimulation. Infection also induced production of mRNA for the beta2 chain of the IL-12 receptor in PP. We conclude that reovirus induces robust Th1 and weak Th2 cell responses in GALT.
用1型呼肠孤病毒对小鼠进行肠道感染会在肠道相关淋巴组织（GALT）中引发抗体和细胞毒性T淋巴细胞。这导致了一种假设，即GALT中会产生辅助性T细胞1（Th1）和辅助性T细胞2（Th2）反应。对派尔集合淋巴结（PP）、上皮内淋巴细胞（IEL）和固有层（LP）淋巴细胞的RNA进行逆转录聚合酶链反应表明，感染后PP和IEL中的干扰素（IFN）-γ信息增加，但LP中未增加。未检测到白细胞介素（IL）-4、IL-5或IL-6的mRNA增加。感染后4-10天，PP的体外培养物中产生了IFN-γ、IL-5和IL-6。感染小鼠的PP和脾淋巴细胞在体外再刺激后产生IFN-γ，但不产生IL-5。感染还诱导了PP中IL-12受体β2链的mRNA产生。我们得出结论，呼肠孤病毒在GALT中诱导强烈的Th1和微弱的Th2细胞反应。
