Blum A M, Metwali A, Elliott D, Li J, Sandor M, Weinstock J V
Division of Gastroenterology-Hepatology, University of Iowa, Iowa City, Iowa 52242, USA.
Cell Immunol. 1998 Aug 25;188(1):64-72. doi: 10.1006/cimm.1998.1351.
IL-6 can play an important role in various biological activities. Using IL-6-deficient, 129 x C57BL/6 mice and normal littermate controls, we studied the role of IL-6 in granulomas of mice infected with schistosomiasis mansoni. Granulomas from IL-6(+/+) mice produced large quantities of IL-6, derived from T, B, and myeloid cells. Yet, IL-6 mutant mice generated normal-appearing granulomas of appropriate size. Multiple-parameter flow cytometric analysis of dispersed granuloma cells revealed no substantial differences. Granuloma cells and splenocytes were cultured in vitro to measure cytokine and immunoglobulin production. Compared to control cells, IL-6(-/-) granuloma cells secreted more IL-4, IL-5, and IL-10. However, splenocytes secreted cytokines comparably. In the IL-6(-/-) state, the granuloma cells released less IgE and substantially more IgM, although IgG1, IgG2a, and IgA secretion remained normal. ELISPOT assay showed that dispersed granuloma cells from IL-6-deficient animals had substantially more IgM-secreting B cells. Thus, schistosome granulomas make IL-6 that is not essential for most aspects of granuloma development. However, IL-6 deficiency results in some disturbance of granuloma cytokine and immunoglobulin expression.
白细胞介素-6（IL-6）在多种生物学活性中发挥重要作用。我们使用白细胞介素-6基因缺陷的129品系与C57BL/6品系杂交小鼠以及正常同窝对照小鼠，研究了IL-6在感染曼氏血吸虫病小鼠肉芽肿中的作用。来自IL-6（+/+）小鼠的肉芽肿产生大量源自T细胞、B细胞和髓样细胞的IL-6。然而，IL-6基因敲除小鼠产生外观正常、大小合适的肉芽肿。对分散的肉芽肿细胞进行多参数流式细胞术分析未发现实质性差异。将肉芽肿细胞和脾细胞进行体外培养以检测细胞因子和免疫球蛋白的产生。与对照细胞相比，IL-6（-/-）肉芽肿细胞分泌更多的IL-4、IL-5和IL-10。然而，脾细胞分泌细胞因子的情况相当。在IL-6（-/-）状态下，肉芽肿细胞释放的IgE较少，而IgM显著增多，尽管IgG1、IgG2a和IgA的分泌仍正常。酶联免疫斑点分析显示，来自IL-6缺陷动物的分散肉芽肿细胞中有更多分泌IgM的B细胞。因此，血吸虫肉芽肿会产生IL-6，而IL-6对肉芽肿发育的大多数方面并非必不可少。然而，IL-6缺乏会导致肉芽肿细胞因子和免疫球蛋白表达出现一些紊乱。
