Pala S, Crimaldi G, Consolini R, Macchia P
Istituto di Clinica Pediatrica, Università di Pisa, Italia.
Pediatr Med Chir. 1998 May-Jun;20(3):213-6.
Natural measles virus infection is recognized for causing prolonged abnormalities in immune responses, that contribute to the severe and complicated evolution of the disease. Immunization with live measles virus vaccine could be considered a mild form of the measles infection. Results of investigation of in vitro immune response after measles immunization with live attenuate vaccine have been conflicting. In this work we studied cellular immune parameters in children aged between 3 and 12 years. T cells, CD4+ and CD8+ subsets were analyzed by cytometry. CD3+ cells were significantly reduced compared to controls (p < 0.01) whereas CD4+/CD8+ ratio was normal. The in vitro proliferative response to polyclonal mitogen was significantly reduced (p < 0.01). This study confirms the presence of a mainly functional immunosuppression of cellular response in a cohort of children belonging to a developed area. These findings improve the understanding of the mechanism of immune response to virus measles and provide suggestions for the development of a better approach to immunization, taking account for the strain, vaccine titre, age and environmental conditions of the target population.
自然感染麻疹病毒会导致免疫反应长期异常，这会促使疾病严重且复杂地发展。接种麻疹活病毒疫苗可被视为一种轻度的麻疹感染形式。关于接种减毒活疫苗后体外免疫反应的研究结果一直存在矛盾。在这项研究中，我们对3至12岁儿童的细胞免疫参数进行了研究。通过细胞计数法分析了T细胞、CD4+和CD8+亚群。与对照组相比，CD3+细胞显著减少（p < 0.01），而CD4+/CD8+比值正常。对多克隆有丝分裂原的体外增殖反应显著降低（p < 0.01）。本研究证实，在一个发达地区的儿童队列中，存在主要是细胞反应的功能性免疫抑制。这些发现有助于加深对麻疹病毒免疫反应机制的理解，并为制定更好的免疫接种方法提供建议，同时考虑目标人群的毒株、疫苗效价、年龄和环境条件。
