Zabaleta E, Heiser V, Grohmann L, Brennicke A
Universität Ulm, Germany.
Plant J. 1998 Jul;15(1):49-59. doi: 10.1046/j.1365-313x.1998.00177.x.
Regulatory promoter regions responsible for the enhanced expression in anthers and pollen are defined in detail for three nuclear encoded mitochondrial Complex I (nCl) genes from Arabidopsis thaliana. Specific regulatory elements were found conserved in the 5' upstream regions between three different genes encoding the 22 kDa (PSST), 55 kDa NADH binding (55 kDa) and 28 kDa (TYKY) subunits, respectively. Northern blot analysis and transgenic Arabidopsis plants carrying progressive deletions of the promoters fused to the beta-glucuronidase (GUS) reporter gene by histochemical and fluorimetric methods showed that all three promoters drive enhanced expression of GUS specifically in anther tissues and in pollen grains. In at least two of these promoters the -200/-100 regions actively convey the pollen/anther-specific expression in gain of function experiments using CaMV 35S as a minimal promoter. These nCl promoters thus contain a specific regulatory region responding to the physiological demands on mitochondrial function during pollen maturation. Pollen-specific motifs located in these regions appear to consist of as little as seven nucleotides in the respective promoter context.
对拟南芥中三个核编码线粒体复合体I（nCl）基因，详细定义了负责花药和花粉中增强表达的调控启动子区域。分别在编码22 kDa（PSST）、55 kDa NADH结合（55 kDa）和28 kDa（TYKY）亚基的三个不同基因的5'上游区域发现了保守的特定调控元件。通过组织化学和荧光法对携带与β-葡萄糖醛酸酶（GUS）报告基因融合的启动子进行渐进缺失的转基因拟南芥植株进行Northern印迹分析，结果表明所有这三个启动子均驱动GUS在花药组织和花粉粒中特异性增强表达。在至少其中两个启动子中，在以CaMV 35S作为最小启动子的功能获得实验中，-200/-100区域积极传递花粉/花药特异性表达。因此，这些nCl启动子包含一个特定的调控区域，可响应花粉成熟过程中线粒体功能的生理需求。位于这些区域的花粉特异性基序在各自的启动子背景下似乎仅由七个核苷酸组成。
