Charpin C, Garcia S, Andrac L, Horschowski N, Choux R, Lavaut M N
Department of Pathology, Timone Faculty of Medecine (IFR Immunologie et Cancérologie), and Hôpital Nord (CHU), Marseille, France.
Hum Pathol. 1998 Sep;29(9):896-903. doi: 10.1016/s0046-8177(98)90193-9.
Expression of vascular cell adhesion molecules (VCAM) in tumors is associated with endothelial cell activation and may facilitate adherence of carcinomatous cells to the vessel wall, promoting bloodborne metastases. Expression of VCAM was investigated in 202 breast carcinomas using automated (Ventana System) and quantitative (SAMBA image analyzer) immunoperoxidase staining of frozen sections. Positive VCAM immunoreactivity was observed in 83 tumors (41%) (mean immunostained surface, 12.4%; SD, 10.5). The mean area of immunostaining was correlated with clinical and pathologic prognostic indicators and with the immunohistochemical expression in tissue sections of various indicators of cell proliferation, metastatic potential, and drug resistance or sensitivity, evaluated according to the same method. There was no correlation of VCAM immunoreactivity with tumor size, type, or grade or with nodal status. Also, no significant correlation was observed between VCAM and MIB1/Ki67, p53, Bcl-2, E cadherin, CD44v, cathepsin D, CD31, P-gp, ER, PR, or pS2. However, VCAM immunoreactivity was significantly correlated with ELAM and VLA2 (P = .001) and VLAs (P = .008) expression. The results suggest that VCAM expression in breast carcinoma tissue sections is likely not a prognostic indicator. Its practical clinical relevance, if any, must be established by correlation with patients' outcomes and tumor sensitivity to drugs.
肿瘤中血管细胞黏附分子（VCAM）的表达与内皮细胞活化相关，可能促进癌细胞黏附于血管壁，从而促进血行转移。采用自动（Ventana系统）和定量（SAMBA图像分析仪）免疫过氧化物酶染色法，对202例乳腺癌冰冻切片中的VCAM表达进行了研究。在83个肿瘤（41%）中观察到阳性VCAM免疫反应性（平均免疫染色面积为12.4%；标准差为10.5）。免疫染色的平均面积与临床和病理预后指标以及根据相同方法评估的细胞增殖、转移潜能和耐药性或敏感性等各种指标在组织切片中的免疫组化表达相关。VCAM免疫反应性与肿瘤大小、类型、分级或淋巴结状态无关。此外，未观察到VCAM与MIB1/Ki67、p53、Bcl-2、E钙黏蛋白、CD44v、组织蛋白酶D、CD31、P-糖蛋白、雌激素受体（ER）、孕激素受体（PR）或pS2之间存在显著相关性。然而，VCAM免疫反应性与ELAM和VLA2（P = 0.001）以及VLA（P = 0.008）的表达显著相关。结果表明，乳腺癌组织切片中的VCAM表达可能不是一个预后指标。其实际临床相关性（如果有的话）必须通过与患者预后及肿瘤对药物的敏感性相关联来确定。
