Endo C, Sagawa M, Sato M, Chen Y, Sakurada A, Aikawa H, Takahashi S, Usuda K, Saito Y, Fujimura S
Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Br J Cancer. 1998 Sep;78(5):612-5. doi: 10.1038/bjc.1998.549.
Radiographically occult bronchogenic squamous cell carcinomas are early lung cancers that localize mainly in the bronchial wall, and are thought to be a good model for investigating genetic alterations through lung cancer progression. In order to elucidate sequential genetic changes in lung cancers, we analysed the incidence of allelic losses on chromosome regions 2q33, 3p21, 5q21, 7q31, 9p21 and 17p13 for 40 cases of radiographically occult bronchogenic squamous-cell carcinomas and 40 cases of advanced lung cancers microdissected. In this study we used eight microsatellite dinucleotide polymorphic markers. Frequent loss of heterozygosity (LOH) was observed on 3p21 (53%), 5q21 (44%) and 17p13 (61%) in roentgenographically occult bronchogenic squamous cell carcinomas. 2q, 7q and 9p were lost less frequently in both roentgenographically occult bronchogenic squamous cell carcinomas and advanced lung cancers. These results suggest that several tumour-suppressor genes are associated with lung cancer progression and that genetic changes on 3p21, 5q21 and 17p13 are early events.
影像学隐匿性支气管源性鳞状细胞癌是主要定位于支气管壁的早期肺癌，被认为是研究肺癌进展过程中基因改变的良好模型。为了阐明肺癌中的序贯基因变化，我们分析了40例影像学隐匿性支气管源性鳞状细胞癌和40例显微切割的晚期肺癌中2q33、3p21、5q21、7q31、9p21和17p13染色体区域的等位基因缺失发生率。在本研究中，我们使用了8个微卫星二核苷酸多态性标记。在影像学隐匿性支气管源性鳞状细胞癌中，3p21（53%）、5q21（44%）和17p13（61%）观察到频繁的杂合性缺失（LOH）。在影像学隐匿性支气管源性鳞状细胞癌和晚期肺癌中，2q、7q和9p的缺失频率较低。这些结果表明，几个肿瘤抑制基因与肺癌进展相关，并且3p21、5q21和17p13上的基因变化是早期事件。
