β-紫罗兰酮对斯普拉格-道利大鼠细胞色素P450s及NADPH-细胞色素P450还原酶表达的影响

Effects of beta-ionone on the expression of cytochrome P450s and NADPH-cytochrome P450 reductase in Sprague Dawley rats.

作者信息

Jeong T C, Gu H K, Chun Y J, Yun C H, Han S S, Roh J K

机构信息

Toxicology Research Center, Korea Research Institute of Chemical Technology, Yusung, Taejon, South Korea.

出版信息

Chem Biol Interact. 1998 Jul 3;114(1-2):97-107. doi: 10.1016/s0009-2797(98)00044-1.

DOI:10.1016/s0009-2797(98)00044-1

PMID:9744558

Abstract

We have recently reported that beta-ionone induces cytochrome P450 (P450) 2B1 in rats. Effects of beta-ionone on the expression of other P450 isozymes and NADPH-P450 reductase were further investigated in Sprague Dawley rats. Administration of beta-ionone subcutaneously 72 and 48 h before sacrificing the animals not only significantly induced the liver microsomal activities of P450-associated enzymes and NADPH-P450 reductase, but also clearly increased in the level of P450 1A1/2, P450 2C, and NADPH-P450 reductase proteins. The induction of P450 1A1/2 and 2C by beta-ionone was much greater in male than in female as measured by western immunoblotting. Reverse transcriptase-polymerase chain reactions showed that, in addition to P450 2B1 and 2B2 mRNAs, P450 1A2, 2C6 and NADPH-P450 reductase mRNAs were increased when beta-ionone was administered. Our previous and present results indicated that beta-ionone may induce several P450s and NADPH-P450 reductase by the accumulation of their corresponding mRNAs.

摘要

我们最近报道了β-紫罗兰酮可诱导大鼠体内细胞色素P450（P450）2B1。在斯普拉格-道利大鼠中进一步研究了β-紫罗兰酮对其他P450同工酶和NADPH-P450还原酶表达的影响。在处死动物前72小时和48小时皮下注射β-紫罗兰酮，不仅显著诱导了P450相关酶和NADPH-P450还原酶的肝微粒体活性，还明显提高了P450 1A1/2、P450 2C和NADPH-P450还原酶蛋白的水平。通过western免疫印迹法测定，β-紫罗兰酮对P450 1A1/2和2C的诱导在雄性大鼠中比雌性大鼠中更强。逆转录聚合酶链反应表明，除了P450 2B1和2B2 mRNA外，给予β-紫罗兰酮时，P450 1A2、2C6和NADPH-P450还原酶mRNA也增加。我们之前和现在的结果表明，β-紫罗兰酮可能通过其相应mRNA的积累来诱导多种P450和NADPH-P450还原酶。